高粱种子醇溶蛋白的提取条件

Journal of Shanghai University（Natural Science Edition） ›› 2007, Vol. 13 ›› Issue (6): 746-750 .

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Different Conditions of Sorghum Seed's Kafirin Extraction

WANG Wei1,YIN Li-ping2,CHEN Qin1

1. School of Life Sciences, Shanghai University, Shanghai 200444, China;
2. Shanghai EntryExit Quarantine and Inspection Bureau, Shanghai 200135, China

Received:2006-09-22 Revised:1900-01-01 Online:2007-12-20 Published:2007-12-20
Contact: WANG Wei1

Abstract

Abstract:

Effects of different reagents, dosages, concentrations, and reduction reagent concentrations on kafirin extraction from -Sorghum bicolor-were investigated. Measurements of protein concentration and the sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE) showed that 60% τbutanol plus 4% DTT at a sample-weight to extraction-solution ratio of 1∶8 (g/mL) was optimum for kafirin extraction. Other sorghum species' alcoholsolute protein (prolamin) were extracted under this condition. It is concluded that, by studying the protein “fingerprint”, different sorghum species can be discriminated from each other.

Key words: extraction, kafirin, sorghum

WANG Wei;YIN Li-ping;CHEN Qin. Different Conditions of Sorghum Seed's Kafirin Extraction[J]. Journal of Shanghai University（Natural Science Edition）, 2007, 13(6): 746-750 .

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Different Conditions of Sorghum Seed's Kafirin Extraction

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