Journal of Shanghai University(Natural Science Edition) ›› 2015, Vol. 21 ›› Issue (2): 237-244.doi: 10.3969/j.issn.1007-2861.2014.03.007

• Metallurgical Materials • Previous Articles     Next Articles

Effect of RNA interference targeting SEMA3A on proliferation and metastasis of glioma cell line U251

HE Zhi-min1, MA Wen-li1,2, ZHENG Wen-ling1,3   

  1. HE Zhi-min1, MA Wen-li1,2, ZHENG Wen-ling1,3
  • Received:2014-03-24 Online:2015-04-29 Published:2015-04-29

Abstract: This paper explores the effect of knockdown SEMA3A gene on the migration and invasion of glioma cell line U251. Preliminary discussion of the potential in treatment of glioma was carried out. The highest knockdown efficiency plasmid packaged by a lentivirus packaging vector system was constructed, which contained a pFH-GFP-L vector and a short hairpin RNA (shRNA) sequence targeting the human SEMA3A gene. U251 cells were infected with a modified lentivirus vector. The infection efficiency of shRNA targeting SEMA3A gene was observed by fluorescence microscope. Real-time PCR reaction and
Western-blot were used to investigate the expression of mRNA and protein of SEMA3A. The results show that the lentivirus containing SEMA3A target shRNA are efficient in silencing SEMA3A expression (P < 0.01). Knockdown SEMA3A gene expression could significantly inhibit the proliferation and metastasis of U251 cells (P < 0.05). In addition,a large number of U251 cells are arrested in G2/M phase, promoting apoptosis (P < 0.05).

Key words: glioma, metastasis, proliferation, SEMA3A gene

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