PinX1基因真核表达载体的构建及其对乳腺癌MCF-7细胞增殖的抑制作用

doi:10.3969/j.issn.1007-2861.2013.06.015

Abstract

Abstract: This paper explores the effects of PinX1 gene on the growth and the cell cycles of breast carcinoma cell line MCF-7 and the potential in treatment of breast cancer. The PinX1 mRNA was amplified from 293-T cell with RT-PCR and inserted into pEGFP-C1 vector. The recombined eukaryotic expression vector of PinX1 was confirmed by enzyme digestion, and transfected into the breast carcinoma cell line MCF-7 using a liposome method. The expressed mRNA was detected by real-time PCR, and the cell growth and cell cycles by MTT and flow cytometry, respectively. Results showed that PinX1 transfection into the breast cancer MCF-7 cell line was stable and the transfected gene was integrated into nucleolus DNA of transfected cells. Transfection of PinX1 gene could significantly inhibit the growth and proliferation of breast cancer cells (P < 0.05) and the cell growth was arrested at G0/G1 stage. The conclusion is that PinX1 gene could inhibit growth and proliferation of breast cancer cells.

Key words: PinX1 gene, breast cancer, cell proliferation, vector construction

CLC Number:

R 736.3

HU Zong1, LIANG Sang-hua2, MA Wen-li1,2, ZHENG Wen-ling1,3. Construction of Recombinant Eukaryotic Expression Vector for PinX1 and Its Roles in Inhibiting Proliferation of Human Breast Cancer MCF-7 Cell Line[J]. Journal of Shanghai University（Natural Science Edition）, 2013, 19(6): 631-635.

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Construction of Recombinant Eukaryotic Expression Vector for PinX1 and Its Roles in Inhibiting Proliferation of Human Breast Cancer MCF-7 Cell Line

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