Numb基因不同亚型对乳腺癌细胞MCF-7增殖和迁移能力的影响

doi:10.12066/j.issn.1007-2861.1822

Abstract

Abstract:

Objective This study explores the role of Numb isoforms in proliferation and migration of breast cancer cells. Methods TALEN technology was used to knock out Numb gene in MCF-7 cells, and then transfected p65, p66, p71 and p72 expression vectors into these Numb-KO MCF-7 cells. On this occasion, CCK-8 proliferation assay and wound scratch assay were used to detect MCF-7 cell proliferation and migration. Results The results show that transcription activator-like effector nuclease (TALEN) is effective in knocking out Numb gene. Compared with normal MCF-7 cells, proliferation and migration abilities of Numb-KO MCF-7 cells are significantly enhanced. Numb isoform p72 inhibits proliferation of Numb-KO MCF-7 cells, and Numb isoforms p65, p66, p71 and p72 inhibit migration of Numb-KO MCF-7 cells.

Key words: breast cancer, Numb gene, isoform, cell proliferation, cell migration

CLC Number:

SU Hang, WANG Baolei, LI Huashun, ZHU Chenguang. Effect of different Numb isoforms on proliferation and migration abilities of breast cancer cell line MCF-7[J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(3): 476-485.

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Effect of different Numb isoforms on proliferation and migration abilities of breast cancer cell line MCF-7

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