梯度有序支架材料诱导神经干细胞的定向迁移

doi:10.12066/j.issn.1007-2861.1894

Journal of Shanghai University（Natural Science Edition） ›› 2019, Vol. 25 ›› Issue (1): 164-170.doi: 10.12066/j.issn.1007-2861.1894

• Research Articles • Previous Articles

Directional migration of neural stem cells induced by orderly gradient scaffolds

LI Mengyuan¹^,²^,³, LI Xiaoran², DAI Jianwu², WEN Tieqiao¹()

1. School of Life Sciences, Shanghai University, Shanghai 200444, China
2. Key Laboratory for Nano-Bio Interfaces Research, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Science, Suzhou 215123, Jiangsu, China
3. Key Laboratory of Hui Ethnic Medicine Modernization Ministry of Education, Ningxia Medical University, Yinchuan 750004, China

Received:2017-03-04 Online:2019-02-28 Published:2019-02-26
Contact: WEN Tieqiao E-mail:wtq@t.shu.edu.cn

Abstract

Abstract:

Stromal-cell-derived factor-1$\alpha $ (SDF1$\alpha )$ plays an important role in inducing stem cell migration. Using a modified electrospinning technique, CBD-SDF1$\alpha $ embedded in radial orderly gradient fiber scaffolds to study migration of neural stem cells (NSCs) and neural stem cell spheres (NSCS) was prepared. The following results were obtained. 1) Gradient scaffolds induced migration of NSCs and NSCS along the orderly fiber scaffolds from a low concentration area to a high concentration area of collagen-binding domain SDF1$\alpha $ (CBD-SDF1$\alpha $). 2) The number and diffusion area in NSCs derived from NSCS were increased significantly in a high concentration area than in a low concentration area of CBD-SDF1$\alpha $. 3) NSCS exhibited polarized morphology and extended directionally along aligned gradient fiber scaffolds.

Key words: neural stem cells (NSCs), neural stem cell sphere (NSCS), cell migration

CLC Number:

LI Mengyuan, LI Xiaoran, DAI Jianwu, WEN Tieqiao. Directional migration of neural stem cells induced by orderly gradient scaffolds[J]. Journal of Shanghai University（Natural Science Edition）, 2019, 25(1): 164-170.

Figures/Tables 6

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References 14

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Directional migration of neural stem cells induced by orderly gradient scaffolds

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