Numb基因不同亚型对乳腺癌细胞MCF-7增殖和迁移能力的影响

doi:10.12066/j.issn.1007-2861.1822

上海大学学报(自然科学版) ›› 2018, Vol. 24 ›› Issue (3): 476-485.doi: 10.12066/j.issn.1007-2861.1822

Numb基因不同亚型对乳腺癌细胞MCF-7增殖和迁移能力的影响

苏杭¹^,², 王保垒³, 李华顺²^,³, 朱晨光¹()

1.上海大学生命科学学院, 上海 200444
2.中国科学院上海高等研究院干细胞与纳米医学研究中心, 上海 201210
3.同济大学附属东方医院转化医学高等研究院, 上海 200085

收稿日期:2016-06-21 出版日期:2018-06-15 发布日期:2018-06-28
通讯作者: 朱晨光 E-mail:cgzhu@shu.edu.cn
基金资助:
国家自然科学基金资助项目(31071283)

Effect of different Numb isoforms on proliferation and migration abilities of breast cancer cell line MCF-7

SU Hang¹^,², WANG Baolei³, LI Huashun²^,³, ZHU Chenguang¹()

1. School of Life Sciences, Shanghai University, Shanghai 200444, China
2. SARI Center for Stem Cell and Nanomedicine, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
3. SARITEX Center for Stem Cell Engineering Translational Medicine, Eastern Hospital Affiliated to Tongji University, Shanghai 200085, China

Received:2016-06-21 Online:2018-06-15 Published:2018-06-28
Contact: ZHU Chenguang E-mail:cgzhu@shu.edu.cn

摘要/Abstract

摘要：

目的探讨Numb基因的4种不同亚型对乳腺癌细胞增殖和迁移能力的影响.方法应用转录激活样效应因子核酸酶(transcription activator-like effector nuclease, TALEN)技术敲除乳腺癌细胞MCF-7中Numb基因, 分别将p65, p66, p71和p72这4种Numb亚型的表达载体转染进Numb-KO MCF-7细胞,应用CCK-8细胞增殖实验、细胞划痕实验、流式细胞术检测Numb基因及其4种亚型对MCF-7胞增殖和迁移能力的影响.结果利用TALEN技术可以有效敲除MCF-7细胞中Numb基因,Numb-KO MCF-7组细胞的增殖和侵袭能力明显高于正常MCF-7细胞,Numb亚型p72对Numb-KO MCF-7细胞的增殖有明显的抑制作用,Numb亚型p65, p66, p71和p72均对Numb-KO MCF-7细胞的迁移有明显抑制作用.

关键词: 乳腺癌, Numb基因, 亚型, 增殖, 迁移

Abstract:

Objective This study explores the role of Numb isoforms in proliferation and migration of breast cancer cells. Methods TALEN technology was used to knock out Numb gene in MCF-7 cells, and then transfected p65, p66, p71 and p72 expression vectors into these Numb-KO MCF-7 cells. On this occasion, CCK-8 proliferation assay and wound scratch assay were used to detect MCF-7 cell proliferation and migration. Results The results show that transcription activator-like effector nuclease (TALEN) is effective in knocking out Numb gene. Compared with normal MCF-7 cells, proliferation and migration abilities of Numb-KO MCF-7 cells are significantly enhanced. Numb isoform p72 inhibits proliferation of Numb-KO MCF-7 cells, and Numb isoforms p65, p66, p71 and p72 inhibit migration of Numb-KO MCF-7 cells.

Key words: breast cancer, Numb gene, isoform, cell proliferation, cell migration

中图分类号:

苏杭, 王保垒, 李华顺, 朱晨光. Numb基因不同亚型对乳腺癌细胞MCF-7增殖和迁移能力的影响[J]. 上海大学学报(自然科学版), 2018, 24(3): 476-485.

SU Hang, WANG Baolei, LI Huashun, ZHU Chenguang. Effect of different Numb isoforms on proliferation and migration abilities of breast cancer cell line MCF-7[J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(3): 476-485.

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Numb基因不同亚型对乳腺癌细胞MCF-7增殖和迁移能力的影响

Effect of different Numb isoforms on proliferation and migration abilities of breast cancer cell line MCF-7

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