上海河流沉积物的有机提取物对3 种细胞的毒性效应

doi:10.3969/j.issn.1007-2861.2013.04.012

上海大学学报(自然科学版) ›› 2013, Vol. 19 ›› Issue (4): 393-399.doi: 10.3969/j.issn.1007-2861.2013.04.012

上海河流沉积物的有机提取物对3 种细胞的毒性效应

康佳1, 雷炳莉1, 刘倩1, 王学彤1, 吴明红1, 徐刚1, 傅家谟1,2

(1. 上海大学环境与化学工程学院, 上海200444;2. 中国科学院广州地球化学研究所, 有机地球化学国家重点实验室, 广州510640)

出版日期:2013-08-27 发布日期:2013-08-27
通讯作者: 雷炳莉(1979—), 女, 副研究员, 博士, 研究方向为环境毒理学与环境风险评价. E-mail: leibingli@126.com
作者简介:雷炳莉(1979—), 女, 副研究员, 博士, 研究方向为环境毒理学与环境风险评价. E-mail: leibingli@126.com
基金资助:
上海市重点学科建设资助项目(S30109); 上海市优秀青年基金资助项目(SHU1004)

Comprehensive Toxicity Effects on Three Cells of Sediment Organic Extractions from Major Rivers in Shanghai

KANG Jia1, LEI Bing-li1, LIU Qian1, WANG Xue-tong1,WU Ming-hong1, XU Gang1, FU Jia-mo1,2

(1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;
2. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry,
Chinese Academy of Sciences, Guangzhou 510640, China)

Online:2013-08-27 Published:2013-08-27

摘要/Abstract

摘要： 河流沉积物是众多有机污染物的最终储存库. 采用索氏抽提法提取出黄浦江及支流苏州河沉积物中的有机污染物, 用CCK-8 法检测沉积物中的有机提取物对正常人肝细胞L02、人肝癌细胞HepG2 以及人乳腺癌细胞MCF-7 的细胞增殖毒性, 综合评价沉积物中有机污染物的综合毒性效应, 进而比较沉积物有机提取物对这3 种细胞增殖毒性的差异及两条河流沉积物受有机污染物污染的情况差异. 结果表明, 黄浦江与苏州河沉积物有机提取物对MCF-7 细胞和L02 及HepG2 细胞表现出不同的毒性效应, 黄浦江与苏州河沉积物中的大部分样品对L02 和HepG2 表现出一定的促增殖效应或没有影响; 对MCF-7 细胞, 除个别样品外, 大部分样品表现出细胞增殖毒性效应, 并且呈剂量效应及时间效应依赖关系, 其毒性大小与采样的位置存在很大关联, 这说明黄浦江及苏州河沉积物有机提取物一定程度上受到了有机污染物的污染. 另外, 两条河流对L02 和HepG2 的促增殖效应和对MCF-7 细胞增殖的毒性效应相当, 说明两条河流受有机污染物的污染强度接近及污染物类型相似.

关键词: CCK-8 法, 毒性效应, 细胞增殖, 有机提取物

Abstract: Sediments are considered a repository of many organic pollutants. In this work, organic pollutants are extracted in the sediments from Huangpu River and Suzhou Creek in Shanghai using Soxhlet. The comprehensive toxicity of the sediment organic extractions to three cell proliferations including normal liver cells L02, hepatoma cells HepG2 and human breast cancer cells MCF-7 cells using the CCK-8 assay is evaluated. Proliferation toxicity differences of the sediment organic extracts on the three cells were compared. The results showed that the sediment organic extractions of Huangpu River and Suzhou Creek exhibited different toxic effects on the three cells. For L02 and HepG2, most samples showed positive cell proliferation effect or no effect; while for MCF-7 cells, majority of the samples have different degree toxic effects on MCF-7 cell proliferation and presented dose-effect dependent and time-effect dependent relationships. Besides, the toxicity effect for MCF-7 cell proliferation had a great relevance with the location of sampling. That Huangpu River and Suzhou Creek sediments were polluted by some organic toxicity pollutants can be concluded. In addition, sediments from the two rivers showed a similar toxic effect on the proliferation of L02, HepG2 and MCF-7 cells, indicating that pollution intensity and types of organic pollutants in the two rivers are similar.

Key words: CCK-8 assay, cell proliferation, organic sediment extraction, toxicity effects

中图分类号:

X 132

康佳, 雷炳莉, 刘倩, 王学彤, 吴明红, 徐刚, 傅家谟. 上海河流沉积物的有机提取物对3 种细胞的毒性效应[J]. 上海大学学报(自然科学版), 2013, 19(4): 393-399.

参考文献

[1] Perira W E, Domagalsi J L, Hostettler F D,

et al. Occurrence and accumulation of pesticides and

organic contaminants in river sediment , water and

clam tissues from the San Joaquin river and tributaries,

California [J]. Environmental Toxicology and

Chemistry, 1996, 15(2): 172-180.

[2] Hong H, Xu L, Zhang L, et al. Environment fate

and chemistry of organic pollutant in the sediment of

Xiamen and Victoria Harbors [J]. Marine Pollution

Bulletin, 1995, 31: 229-236.

[3] Strategic Environmental Research and Development

Program. SERDP and ESTCP Expert Panel Workshop

on research and development needs for understanding

and assessing the bioavailability of contaminants

in soils and sediment [R]. SERDP and ESTCP

Expert Panel Worshop on Research, USA. Workshop

Report, November, 2008.

[4] 牛菲, 安娜, 李慧岩, 等. 松花江水源水中有机提取物诱

导MCF-7细胞增殖的研究[J]. 环境与健康杂志, 2010,

27(6): 482-484.

[5] 丁国武, 魏延萍, 牛静萍, 等. 黄河兰州段水体中有机污

染物对MCF-7细胞凋亡的影响及其机制[J]. 兰州大学

学报: 自然科学版, 2012, 48(2): 91-96.

[6] Wu M H, Wang L, Xu G, et al. Seasonal and spatial

distribution of 4-tert -octylphenol,4-nonylphenol and

bisphenol A in the Huangpu River and its tributaries,

Shanghai, China [J]. Environmental Monitoring and

Assessment, 2012, 12: 2779-2785.

[7] 吴明红, 李刚, 徐刚, 等. 上海市河流表层沉积物中的

氯酚、溴酚及多环芳烃[J]. 环境化学, 2012, 31(11):

1750-1758.

[8] 董煜, 邹联沛, 钱光人, 等. 苏州河上海市区段表层沉

积物中多环芳烃的分布及特征[J]. 环境污染与防治,

2007, 29(4): 312-315.

[9] 邓涛, 申一尘, 王绍祥, 等. 黄浦江原水中阿特拉津农药

类微污染预警研究[J]. 给水排水, 2009, 35(11): 32-36.

[10] 李康, 戎志毅, 贾丽娟, 等. 苏州河沉积物中的PCDD/

Fs 研究[J]. 环境科学与技术, 2009, 32(9): 55-60.

[11] 曾祥英, 张晓岚, 钱光人, 等. 苏州河沉积物中多环麝香

分布特点的初步研究[J]. 环境科学学报, 2008, 28(1):

180-184.

[12] 魏荣菲, 庄舜尧, 杨浩, 等. 苏州河网区河道沉积物重金

属的污染特征[J]. 湖泊科学, 2010, 22(4): 527-537.

[13] Cunninghan V, Binks S P, Olson M I. Human

health risk assessment from the presence of huaman pharmaceuticals in the aquatic environment [J]. Regulatory

Toxicology and Pharmacology, 2009, 53: 39-

45.

[14] Richardson S D, Ternes T A. Water analysis:

emerging contaminants and current issues [J]. Analytical

Chemisty, 2005, 77(12): 3807-3838.

[15] 景欣悦, 康维钧, 张宏伟. 环境污染物的生物测试方法

[J]. 国外医学卫生学分册, 2005, 32(3): 167-170.

[16] Luo J P, Lei B L, Ma M, et al. Identification of estrogen

receptor agonists in sediments fromWenyu River,

Beijing, China [J]. Water Research, 2011, 45(13):

3908-3914.

[17] Avocet C. Development of fresh water sediment

quality values for use in Washington State. Phase II Report: Development and recommendations of SQVs

For fresh water sediments in Washington State [R].

Prepared for Washington Department of Eeology.

Publieation No. 03-09-088, 2003.

[18] 陈云增, 杨浩, 张振克. 淡水沉积物环境质量基准差异

分析[J]. 湖泊科学, 2005, 17(3): 193-201.

[19] 方宇翘, 漆德瑶. 苏州河的污染底泥评价[J]. 上海环境

科学, 1993(12): 27-30.

[20] 李洋, 胡雪峰, 王效举, 等. 苏州河底泥3 种内分泌干扰

物的空间分布及环境风险[J]. 环境科学, 2012, 33(1):

239-246.

[21] 刘克明, 周蕾, 王春花, 等. 再生水有机提取物雌激素效

应研究[J]. 环境与健康杂志, 2005, 22(3): 174-176.

上海河流沉积物的有机提取物对3 种细胞的毒性效应

Comprehensive Toxicity Effects on Three Cells of Sediment Organic Extractions from Major Rivers in Shanghai

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