新生大鼠心脏再生模型的改良及评价模式

doi:10.12066/j.issn.1007-2861.1938

上海大学学报(自然科学版) ›› 2017, Vol. 23 ›› Issue (3): 387-394.doi: 10.12066/j.issn.1007-2861.1938

新生大鼠心脏再生模型的改良及评价模式

朱浩¹, 丁胜光¹, 黄海涛¹, 许嘉鸿², 仲崇俊¹

1. 南通大学第二附属医院心胸外科, 江苏南通 226001;
2. 同济大学医学院附属同济医院心内科, 上海 200065

收稿日期:2017-04-03 出版日期:2017-06-30 发布日期:2017-06-30
通讯作者: 仲崇俊(1962—), 男, 主任医师, 博士, 研究方向为心脏再生. E-mail: chongjunzhong@hotmail.com
作者简介:仲崇俊(1962—), 男, 主任医师, 博士, 研究方向为心脏再生. E-mail: chongjunzhong@hotmail.com
基金资助:
国家自然科学基金资助项目(81670362, 81470515); 上海市科委医学引导(重点)基金资助项目(134119a3000)

Improvement and preliminary evaluation of neonatal rat cardiac regeneration model

ZHU Hao¹, DING Shengguang¹, HUANG Haitao¹, XU Jiahong², ZHONG Chongjun¹

1. Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China;
2. Department of Cardiology, Tongji Hospital Affiliated to Medical College of Tongji University, Shanghai 200065, China

Received:2017-04-03 Online:2017-06-30 Published:2017-06-30

摘要/Abstract

摘要：

探索改良新生大鼠心脏再生模型并进行初步评价, 以优化哺乳动物在体心脏再生模型. 取出生12 h左右的大鼠, 采用低温麻醉行心尖切除改良术, 术后统计1, 3, 7和21 d存活率, 并取材测量切除的心尖横截面积来评估改良术的效果. 术后1 d对照组存活率为61%, 改良组为77%, 并且改良组术后3, 7, 21 d的存活率都高于对照组. 改良组心尖切除的横截面积变异较小, 说明新生大鼠心脏再生模型改良术均一稳定, 手术成功率高, 可用于哺乳动物心脏再生相关基因和信号通路的研究.

关键词: 细胞增殖, 心尖切除, 心脏再生

Abstract:

An improved neonatal rat cardiac regeneration model was established and a preliminary evaluation was conducted. Neonatal rats of 12 h and exercised apical resection surgery with low-temperature anesthesia were used. Postoperative survival rates were taken on 1, 3, 7 and 21 d, and the cross-sectional area were measured to evaluate the advantage of the improved technique. In the control group, postoperative survival rate at 1 d was 61%, while the improved group was 77%. In addition, survival rates of the improved group on 3, 7 and 21 d were higher than that in the control group. Variation of resected apex crosssectional area was smaller in the improved group. In conclusion, the improved neonatal rat cardiac regeneration model is more stable and more uniform with a higher operation
success rate. This model may be used to investigate genes and the signaling pathways mediating mammalian heart regeneration.

Key words: apical resection, cell proliferation, heart regeneration

朱浩1, 丁胜光1, 黄海涛1, 许嘉鸿2, 仲崇俊1. 新生大鼠心脏再生模型的改良及评价模式[J]. 上海大学学报(自然科学版), 2017, 23(3): 387-394.

ZHU Hao1, DING Shengguang1, HUANG Haitao1, XU Jiahong2, ZHONG Chongjun1. Improvement and preliminary evaluation of neonatal rat cardiac regeneration model[J]. Journal of Shanghai University（Natural Science Edition）, 2017, 23(3): 387-394.

参考文献

[1] Moran A E, Forouzanfar M H, Roth G, et al. Temporal trends in ischemic heart disease mortality in 21 world regions, 1980—2010: the Global Burden of Disease 2010 Study [J]. Circulation, 2014, 129(14): 1483-1492.
[2] Nabel E G, Braunwald E. A tale of coronary artery disease and myocardial infarction [J]. New England Journal of Medicine, 2012, 366(1): 54-63.
[3] Bergmann O, Bhardwaj R D, Bernard S, et al. Evidence for cardiomyocyte renewal in humans [J]. Science, 2009, 324(5923): 98-102.
[4] Forbes S J, Rosenthal N. Preparing the ground for tissue regeneration: from mechanism to therapy [J]. Nature Medicine, 2014, 20(8): 857-869.
[5] Garbern J C, Lee R T. Cardiac stem cell therapy and the promise of heart regeneration [J]. Cell Stem Cell, 2013, 12(6): 689-698.
[6] Poss K D, Wilson L G, Keating M T. Heart regeneration in zebrafish [J]. Science, 2002, 298(5601): 2188-2190.
[7] Jopling C, Sleep E, Raya M, et al. Zebrafish heart regeneration occurs by cardiomyocyte dedifferentiation and proliferation [J]. Nature, 2010, 464(7288): 606-609.
[8] Kikuchi K, Holdway J E, Werdich A A, et al. Primary contribution to zebrafish heart regeneration by gata4(+) cardiomyocytes [J]. Nature, 2010, 464(7288): 601-605.
[9] Li F, Wang X, Capasso J M, et al. Rapid transition of cardiac myocytes from hyperplasia to hypertrophy during postnatal development [J]. Journal of Molecular and Cellular Cardiology,
1996, 28(8): 1737-1746.
[10] Walsh S, Pont´en A, Fleischmann B K, et al. Cardiomyocyte cell cycle control and growth estimation in vivo—an analysis based on cardiomyocyte nuclei [J]. Cardiovascular Research,
2010, 86(3): 365-373.
[11] Hsieh P C H, Segers V F M, Davis M E, et al. Evidence from a genetic fate-mapping study that stem cells refresh adult mammalian cardiomyocytes after injury [J]. Nature Medicine, 2007, 13(8): 970-974.
[12] Pasumarthi K B, Nakajima H, Nakajima H O, et al. Targeted expression of cyclin D2 results in cardiomyocyte DNA synthesis and infarct regression in transgenic mice [J]. Circulation
Research, 2005, 96(1): 110-118.
[13] Quaini F, Urbanek K, Beltrami A P, et al. Chimerism of the transplanted heart [J]. New England Journal of Medicine, 2002, 346(1): 5-15.
[14] Ahuja P, Sdek P, MacLellanWR. Cardiac myocyte cell cycle control in development, disease, and regeneration [J]. Physiological Reviews, 2007, 87(2): 521-544.
[15] Martin-Rendon E, Brunskill S J, Hyde C J, et al. Autologous bone marrow stem cells to treat acute myocardial infarction: a systematic review [J]. European Heart Journal, 2008,
29(15): 1807-1818.
[16] Marbán E, Malliaras K. Mixed results for bone marrow-derived cell therapy for ischemic heart disease [J]. JAMA, 2012, 308(22): 2405-2406.

[17] Yi B A, Wernet O, Chien K R. Pregenerative medicine: developmental paradigms in the biology of cardiovascular regeneration [J]. The Journal of Clinical Investigation, 2010, 120(1):
20-28.
[18] Smart N, Bollini S, Dub´e K N, et al. Myocardial regeneration: expanding the repertoire of thymosin b4 in the ischemic heart [J]. Annals of the New York Academy of Sciences, 2012,
1269(1): 92-101.
[19] Qian L, Huang Y, Spencer C I, et al. In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes [J]. Nature, 2012, 485(7400): 593-598.
[20] Senyo S E, Steinhauser M L, Pizzimenti C L, et al. Mammalian heart renewal by pre-existing cardiomyocytes [J]. Nature, 2013, 493(7432): 433-436.
[21] Makkar R R, Smith R R, Cheng K E, et al. Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): a prospective, randomised phase 1 trial [J]. The Lancet, 2012, 379(9819): 895-904.
[22] Porrello E R, Mahmoud A I, Simpson E, et al. Transient regenerative potential of the neonatal mouse heart [J]. Science, 2011, 331(6020): 1078-1080.
[23] Haubner B J, Adamowicz-Brice M, Khadayate S, et al. Complete cardiac regeneration in a mouse model of myocardial infarction [J]. Aging (Albany NY), 2012, 4(12): 966-977.
[24] Konfino T, Landa N, Ben-Mordechai T, et al. The type of injury dictates the mode of repair in neonatal and adult heart [J]. Journal of the American Heart Association, 2015, 4(1): e001320.
[25] Sen S, Sadek H A. Neonatal heart regeneration: mounting support and need for technical standards [J]. Journal of the American Heart Association, 2015, 4(1): e001727.

新生大鼠心脏再生模型的改良及评价模式

Improvement and preliminary evaluation of neonatal rat cardiac regeneration model

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价

[1]	胡士军1,2, 虞游1,2, 方星1,2, 雷伟1,2, 赵振奥1,2. 诱导多能干细胞与心脏再生[J]. 上海大学学报(自然科学版), 2016, 22(3): 285-292.
[2]	贝毅桦, 肖俊杰. 运动诱导心脏再生: 治疗心血管疾病的新途径[J]. 上海大学学报(自然科学版), 2016, 22(3): 293-301.
[3]	高峰1,2, 陈静海1,2. 非编码RNA调控心脏重构与再生[J]. 上海大学学报(自然科学版), 2016, 22(3): 302-309.
[4]	肖俊杰. 心脏重构与再生: 心血管研究热点聚焦[J]. 上海大学学报(自然科学版), 2016, 22(3): 261-264.
[5]	胡宗1, 梁桑华2, 马文丽1,2, 郑文岭1,3. PinX1基因真核表达载体的构建及其对乳腺癌MCF-7细胞增殖的抑制作用[J]. 上海大学学报(自然科学版), 2013, 19(6): 631-635.
[6]	康佳, 雷炳莉, 刘倩, 王学彤, 吴明红, 徐刚, 傅家谟. 上海河流沉积物的有机提取物对3 种细胞的毒性效应[J]. 上海大学学报(自然科学版), 2013, 19(4): 393-399.