2018 Vol.24

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    Treatment of Chinese Adult Patients with Hypertrophic Cardiomyopathy
    ZOU Yubao, HUI Rutai, SONG Lei
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 1-8.   DOI: 10.12066/j.issn.1007-2861.1999
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    This article introduces the contents of the recently published Guidelines for Diagnosis and Treatment for Chinese Adult Patients with Hypertrophic Cardiomyopathy, which tend to offer a standardized diagnosis and treatment and improved prognosis of patients.

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    Interpretation of Experts' Consensus for Exercise Rehabilitation of the Post-PCI Treatment
    HU Shugang, WANG Lei, GUO Lan
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 9-15.   DOI: 10.12066/j.issn.1007-2861.2000
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    Nowadays, percutaneous coronary intervention (PCI) has become a predominant important method for revascularization in patients with coronary heart disease. China has stepped into the center of global invention research. Nevertheless, many patients still face problems after PCI, such as hypokinesis, emotional anxiety and declining quality of life. Cardiac rehabilitation plays an important role in the therapy and secondary prevention of coronary heart disease, and it has a significant effect on improving above-mentioned postoperative problems of patients. In order to standardize and guide the PCI postoperative rehabilitation, the Cardiovascular Prevention and Rehabilitation Committee of the Chinese Medical doctor Association (CMDA) organized a group of experts of cardiology and rehabilitation to write Experts' Consensus for Exercise Rehabilitation of the Post-PCI Treatment according to the practical situation of China. Its main contents are interpreted in this article.

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    Interpretation of 2014 ESC Guidelines on Diagnosis and Management of Hypertrophic Cardiomyopathy
    ZOU Changhong, ZHAO Xuemei, ZHANG Yuhui
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 16-20.   DOI: 10.12066/j.issn.1007-2861.2005
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    2014 European Society of Cardiology (ESC) Guidelines on Diagnosis and Management of Hypertrophic Cardiomyopathy (HCM) was published in August, 2014, during the congress of ESC 2014. The guideline focused on the definition, epidemiology, etiology,diagnosis and clinical evaluation, genetic testing and family screening, treatments, follow-up, reproduction and contraception associated with HCM. A brief interpretation of these contents is made in the present article.

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    Isolation and identification of mouse hepatic telocytes
    WANG Fei, SONG Meiyi, XIA Lu, ZHANG Zhaojie
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 21-26.   DOI: 10.12066/j.issn.1007-2861.2006
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    [Objective] To develop an optimized method which is more stable and efficient in hepatic telocytes separation based on current method, and to establish a solid theoretical foundation and experimental support for further study of potential regulation of hepatic cells by telocytes. [Methods] C57BL/6 male wild mice were selected for isolation of liver telocytes through multiple short enzymatic digestions and centrifugation. CD34+Vimentin immunofluorescence staining was used to identify the cell phenotype. [Results] Hepatic telocytes were successfully separated. Hepatic telocytes were both CD34 and Vimentin positive in vitro, while hepatic stellate cell line was CD34 negative and Vimentin positive. [Conclusion] High-quality telocytes were obtained through this optimized method and could be used for other studies.

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    Refractive index characteristics and mode splitting of long-period fiber gratings written in the tapered fiber
    ZHOU Chao, SHI Lei, ZOU Fang, LIU Yunqi
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 27-32.   DOI: 10.12066/j.issn.1007-2861.1778
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    Transmission characteristics of long-period fiber gratings (LPFGs) written in a tapered fiber is investigated by numerical simulation. It has been found that splitting of the mode distribution curve happens for the same order cladding mode of LPFG when the thinnest diameter of the tapered fiber is between 55 $\mu$m and 110 $\mu$m. The two resonance dips correspond to splitting from the same order cladding mode show different refractive index characteristics.

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    Dexterous robotic hand control based on motion mapping
    JIANG Caijun, XU Yulin, DING Meikun
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 33-43.   DOI: 10.12066/j.issn.1007-2861.1833
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    A control system for dexterous robotic hands based on motion mapping of data glove is presented in this paper. Humanoid gestures and grasping motion can intuitively be realized on robotic hands through human hand motion mapping by data glove. Operation performance of the robotic hand is improved. Direct joint angle mapping and fingertip position mapping are adapted to control the robotic hand motion. Experimental results show practicality of the control system.

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    Fabrication and biological properties of micro/nano hybrid structured TiO2 coatings
    GU Jingping, XIE Youtao, HUANG Liping, HE Yanchang, ZHAO Jun, ZHENG Xuebin, LI Hong, SUN Jinliang
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 44-55.   DOI: 10.12066/j.issn.1007-2861.1921
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    Micro/nano hybrid structured surface can improve their capability of encouraging early cell responses and rapid bone in-growth. The micro/nano hybrid structured TiO$_{2}$ coatings were prepared by induction suspension plasma spraying (ISPS) using anatase TiO$_{2}$ suspension as a feedstock. Their osteogenesis related cell behaviors were studied. Scanning transmission electron microscopy (SEM) results show that the coatings prepared by adjusting feed rate have typical micro/nano hybrid structures. Porosity of the coating increases with the increase of feed rate. The X-ray diffraction (XRD) results show that the coating is mainly composed of rutile with a small amount of anatase, and the content of rutile decreased with the increase of feed rate. This may be related to the high temperature process of plasma spraying, in which the anatase phase is transformed into a more stable rutile phase. The results of in vitro cell compatibility tests show that the micro/nano structured TiO$_{2}$ coatings prepared with ISPS has higher protein adsorption, better osteoblasts spreading and higher expression of alkaline phosphatase (ALP) activity and mineralization than atmospheric plasma sprayed coatings and polished titanium surfaces.

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    Effects of Y$_{2}$O$_{3}$ on microstructure and properties of zirconia ceramics
    FENG Yajun, WANG Xingqing
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 56-65.   DOI: 10.12066/j.issn.1007-2861.1774
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    The influence of addition of Y$_{2}$O$_{3}$ on microstructures and properties of zirconia ceramics was studied. The influence of ball milling time and the amount of added Y$_{2}$O$_{3 }$ on grain size, microstructures and properties of ZrO$_{2}$ is discussed. The results show that grains of ZrO$_{2 }$ and Y$_{2}$O$_{3 }$ can be refined, and the grain size of zirconia ceramic reduced, leading to improved mechanical properties of ceramics. Fine-grained 3Y-ZrO$_{2}$ ceramics can be prepared by appropriate addition of Y$_{2}$O$_{3 }$ and a common sintering process with good mechanical properties, that is, Vickers hardness and fracture toughness values being 1 128 kg/mm$^{2}$ and 10.03 MPa$\cdot $m$^{1/2}$, respectively.

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    Synthesis and self-assembly of Boc-protected bisguanidines
    ZOU Xiaoliang, GU Jun, SUN Yuren, QIAN Qun
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 66-73.   DOI: 10.12066/j.issn.1007-2861.1768
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    Guanidinium cations are widely used to recognize sulfate and phosphate anions. However, there are few reports about neutral guanidines. In this work, several Boc-protected guanidines are synthesized. With nuclear magnetic resonance (NMR) dilution experiments and single crystal X-ray diffraction. It has been found that there are intermolecular interaction among neutral bisguanidinium in solution and solid state as well.

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    Reaction of 1-chloro-3-buten-2-one with N-acetylcysteine
    LIU Boliang, ZHANG Xinyu
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 74-82.   DOI: 10.12066/j.issn.1007-2861.1846
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    1-Chloro-3-buten-2-one (CBO) is an in vitro metabolite of 1,3-butadiene, a carcinogenic pollutant.CBO is cytotoxic and genotoxic, and can readily form DNA adducts and glutathione (GSH) conjugates. CBO-GSH conjugates are expected to be biotransformed into CBO-N-acetylcysteine (NAC) ones in the kidney, which are excreted in the urine. Thus, CBO-NAC conjugates can be used as biomarkers. However, the reaction of CBO with NAC has not been investigated yet. In the present study, this reaction under in vitro physiological conditions (pH=7.4, 37 ℃) was investigated and two products, which were structurally characterized by 1D/2D nuclear magnetic resonance (NMR) spectroscopy as 1-chloro-4-(N-acetyl-S-cysteinyl)-2-butanone and 1,4-bis(N-acetyl-S-cysteinyl)-2-butanone, were observed. The latter is a novel compound. Identification of the two CBO-NAC conjugates will help future investigation on the in vivo formation of CBO.

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    Photo-initiated disulfide-ene reaction
    LIU Guangyao, MIAO Miao, AN Zesheng
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 83-91.   DOI: 10.12066/j.issn.1007-2861.1759
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    Click chemistry has been widely developed and applied to various fields such as polymers, chemical synthesis, and biology. In recent years, thiol-ene reaction as a type of click chemistry has attracted much attention because it is highly efficient and can be photo-initiated without using toxic metal catalysts. In this paper, the method of photo-initiated disulfide-ene chemistry is introduced. To avoid short-comings of thiol-ene chemistry such as ease of oxidation of thiols and various side reactions, photo-ininitated reactions between disulfides and enes as potential and alternative reactions were studied. Experimental data showed that the reaction rate and yield were affected by various reaction conditions such as oxygen, photo-initiators, different reactants and solvents.

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    Comprehensive risk assessment of ammonia desulfurization for coal-fired flue gas
    MA Zhen, WAN Hao
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 92-99.   DOI: 10.12066/j.issn.1007-2861.1779
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    According to the status of ammonia desulfurization for coal-fired flue gas, a comprehensive risk index system is established by combining a fuzzy assessment method with analytic hierarchical process (AHP) method. A hierarchical structure is established. Judgement matrix is constructed based on calibration of each element and calculated values of elements in all levels. The evaluation system contains five primary factors: technical risk, environmental risk, safety risk, benefit risk and management risk. There are four specific analytical objects for each index. According to the results of experts voting and fuzzy evaluation matrix, risk value can be calculated with reference to the score collection. The result shows that the risk value of ammonia desulfurization process is 60.65, and the risk value of the industry is a little high. Desulfurization technology is on a high risk level, in other words, the risk mainly comes from technology. The result of comprehensive risk assessment is in accordance with the status, and it has a reference value in studying the theory and practice of ammonia desulfurization.

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    Finite element analysis on flexural performance of RC beams strengthened with pre-stressed near-surface-mounted CFRP strips
    ZHANG Zhimei, XIONG Hao
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 100-107.   DOI: 10.12066/j.issn.1007-2861.1807
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    Flexural performance of reinforced concrete (RC) beams strengthened with near-surface-mounted pre-stressed carbon fiber reinforced polymer/plastic (CFRP) strips is analyzed using the finite element software ABAQUS. Correctness of the model is shown by simulating five RC beams strengthened with near-surface-mounted pre-stressed CFRP strips and comparing the results with test. Then the finite element model is used for deep analysis of the flexural performance of RC beams. The beams are affected by different factors such as the pre-stress level of CFRP, amount of CFRP reinforcement, and longitudinal reinforcement ratio and concrete strength grade. Results of the analysis show that the pre-stress level of CFRP and the amount of CFRP reinforcement have greater influence on the cracking load of RC beams. The longitudinal reinforcement ratio has greater influence on the bearing capacity of RC beams. Strength grade of the concrete has some influence on the flexural performance of RC beams, but not as obvious as other factors.

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    Time effect of shear strengths of unsaturated fly-ash and its prediction
    WANG Sicong, SUN Dean
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 108-117.   DOI: 10.12066/j.issn.1007-2861.1803
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    Fly-ash is an industrial waste material, and is often used as a filling material in subgrade engineering. Many factors affect engineering properties of fly-ash. To investigate the effect of water content and age on the strength of fly-ash, a series of direct shear tests on a fly-ash are carried out. Relations between water content or age and shear strength with the same dry density were obtained. The results show that the shear strength and cohesion of the fly-ash change with variation of the saturation degree and increase with age, while the internal friction angle changes a little. Finally, shear strength of unsaturated fly-ash is predicted using unsaturated soil mechanics and soil-water characteristic curve (SWCC).

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    Micro-mechanism analysis of interface behavior of reinforced sand cushion with denti-inclusions
    ZHANG Jun, LIN Yongliang
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 118-125.   DOI: 10.12066/j.issn.1007-2861.1797
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    To investigate the micro-mechanism of interaction between denti-inclusions and sand cushion, elliptical group particles were developed. Numerical simulation of pull-out tests was performed by PFC2D. The model was validated by comparing the numerical results and the pull-out test results. Evolution of the shear band and load transfer mechanisms was studied. The results show that the stress concentration occurs near denti-inclusions, and the upper and lower interfaces are not symmetrical. The shear bond's thickness of sand cushion reinforced with denti-inclusions is significantly larger than clay reinforcement in case of the same pullout displacement. Every denti-inclusion has a different rate of contribution to pull-out force and change when drawing.

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    Establishment of molecular-based high-throughout screening model for histone lysine methyltransferase DOT1L inhibitors
    XU Wei, SU Mingbo, ZHOU Yubo
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 126-133.   DOI: 10.12066/j.issn.1007-2861.1757
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    DOT1L is an epigenetic enzyme that can catalyze methyltransfer activity at histone 3 lysine 79 (H3K79). It is also found to be the first lysine methyltransferase without a SET domain. Research results show that methylation level of H3K79 can influence the expression of some genes, closely related to the development of acute myelocytic leukemia (AML). This work establishes a molecular-based high-throughout screening model for discovering novel DOT1L inhibitors. EPZ-5676 was used as positive control to optimize activity assay and ensure appropriate concentration of enzyme and substrate, and reaction time, etc. The assay was optimized, and the $Z'$-factor was about 0.54, indicating that a molecular-based high-throughout screening model for DOT1L inhibitors was successfully established.

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    Effects of ABL silencing combined with TRAIL and DOX on apoptosis of colorectal cancer HT29 cells
    LI Yuyu, JIN Youxin, XU Zhongjuan, ZHANG Shumang, SUO Guangli
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 134-141.   DOI: 10.12066/j.issn.1007-2861.1771
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    To investigate the effects of Abelson (ABL) tyrosine kinase silencing combined with tumor necrosis factor related apoptosis-inducing ligand (TRAIL) and doxorubicin (DOX) on apoptosis of human colon cancer cell line HT29. ABL was stably knocked down in HT29 cells using lent ivirus particles. The expression level was verified by Western blot. Cell proliferation and drug toxicity was detected by cell count kit 8 (CCK8) assay. Cell apoptosis was detected by flow cytometry. The expression level PARP1 and cleaved-caspase3 were determined by Western blot. The results showed that cell viability was reduced after treatment of TRAIL or DOX. Combination therapy can induce apoptosis rate at a lower dose. The colon cancer cell proliferation was inhibited by ABL gene silencing. However, apoptosis induced by TRAIL was inhibited, and the synergistic apoptotic effect of TRAIL and DOX on HT29 cell was also inhibited. Combination treatment can significantly induce HT29 cell apoptosis. Although ABL silencing can inhibit cell proliferation, the synergistic apoptotic effect of TRAIL and DOX on colorectal cancer HT29 cell was still inhibited by ABL silencing.

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    Construction of cationic amphipathic peptide-lipid nanoparticle and evaluation of its cytotoxicity and stability in vitro
    WANG Qiao, FEI Hao
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (1): 142-150.   DOI: DOi:10.12066/j.issn.1007-2861.1758
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    In this paper, cationic amphipathic R peptide (RLARLLRRLARWLR) was chosen to construct peptide-lipid nanoparticle. Recombination and interaction of R peptide with lipid mixture were studied using lipid emulsion clearance assay and blue shift of tryptophan fluorescence assay. The diameter and surface characterization of cationic amphipathic R peptide-lipid nanoparticle (R-LNP) were obtained with transmission electron microscope (TEM), dynamic light scattering (DLS) and zeta potential measurements. MTT assay was used to investigate cytotoxicity of R-LNP. Stability of R-LNP and R peptide against chymotrypsin and trypsin was explored using ultra high performance liquid chromatography (UPLC). The results showed that R peptide caused reconstruction of lipid to form lipid nanoparticle with a diameter of (14.7$\pm $0.1) nm and zeta potential of 17.8 mV. Hydrophobic amino acids of R peptide inserted in the hydrophobic core of lipid nanoparticle. Cytotoxicity of R peptide was retained in R-LNP, of which half-inhibitory concentration for A549 and MCF-7 was (5.93$\pm $0.75) $\mu$mol/L and (4.36$\pm $0.40) $\mu$mol/L respectively. UPLC results showed that R-LNP increased stability of R peptide, facilitating its translational application in vivo.

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    Pulling memory mapping theory and experiment
    LI Kaikai, JIN Yi, OUYANG Shan, ZHOU Shiqiang
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 151-165.   DOI: 10.12066/j.issn.1007-2861.1899
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    Dual-space memory is a new type of storage system invented in 2013. The key is to map the memory space of CPU to the huge dual-space memory, called the pulling memory mapping theory. This paper proposes the first physical equipment of the dual-space memory and implementation of the pulling memory mapping technique. Design of an experimental system including the dual-space memory and latch group is discussed, and a practical experiment is completed. An operation for mapping the memory space to the huge dual-space memory is achieved on an experimental system. Correctness of the proposed technique is shown in mapping operations. By solving the problem of connecting memory space to the huge dual-space memory, a foundation for building dual-space memories has been laid and the pulling memory mapping technique has been used. The experiment system is made of a TQ2440 Core 2.0 core board, a 1 GB dual-space memory and a latch group consisting of eight 12-bit latches. The core board has 2 MB internal memory space. All access to the 2 MB internal memory space are converted automatically to a local range of a 1 GB dual-space memory by one of the eight 12-bit latches. The local range can be moved in the 1 GB dual-space memory by a pushing command.

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    Communication system of ternary optical computer decoder
    YANG Jialong, JIN Yi, OUYANG Shan, ZHOU Shiqiang
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 166-180.   DOI: 10.12066/j.issn.1007-2861.1898
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    This paper proposes a method of establishing a communication subsystem between a decoder and other units in a ternary optical computer (TOC). Mechanisms of repeatedly sending data and restarting the communication system are used to improve fault-tolerant ability against occasional transient faults of the system and the peripheral devices. Additional identification information is used to detect permanent failures of the communication link. Combining these techniques with inter-integrated circuit (IIC), a practical communication system for TOC decoders is completed. The overall performance of the system is tested in details. Selection of experimental contents, design of experimental software, settings of test cases and the experimental process are discussed. The experimental results show effectiveness and reliability of the communication subsystem.

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    Initial SZG file generation software for ternary optical computer
    LI Shuang, JIN Yi, LIU Yuejun, ZHOU Shiqiang
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 181-191.   DOI: 10.12066/j.issn.1007-2861.1979
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    SZG file is a programming platform for using a ternary optical computer (TOC) with a high-level programming language. SZG file's functions are similar to those of OpenACC in using GPU and MIC, but both are completely different in programming idea and implementation. More importantly, an SZG file is the only way for programmers to present requirements of grouping the processor's bit number and reconfiguring the processor's hardware functions. The method based on SZG file is easy to be understood, and convenient to be used. This paper proposes an initial SZG file generation theory, an algorithm and a generation software. The proposed software can implement some functions such as storing initial SZG file and appending operands to a SZG file. Moreover, the system can store the operands every a few minutes via setting a timer. Therefore, in applying the software, there is no need to understand the SZG file format and the TOC's work process. Users can generate a correct SZG file. Furthermore, in application, TOC can process large amounts of raw data in parallel using the SZG file. Finally, a white box technique is used to test the software. As an example, the software is used by creating an initial SZG file containing two simple calculators. The results confirm validity and correctness of the software.

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    Qiliqiangxin attenuates doxorubicin induced cardiac dysfunction in mice
    WU Xiaodong, JIANG Huimin, YAO Wenming, GAO Rongrong, LI Xinli
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 192-197.   DOI: 10.12066/j.issn.1007-2861.2015
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    To investigate the therapeutic effects of Qiliqiangxin (QLQX) in doxorubicin (DOX)-induced cardiac dysfunction in vivo. Male mice aged 7~9 weeks were randomly divided into several groups. DOX (4 mg/kg) was injected to induce cardiotoxicity via intraperitoneal administration weekly, and QLQX was given by gavage every day for 4 weeks at a dose of 0.5 g/(kg$\cdot$d). After 4 weeks, echocardiography was performed to detect the cardiac function and it was showed that cardiac function could be significantly improved in mice treated with QLQX versus the saline. These findings indicated that QLQX could function as an intervening treatment for the patients who were treated with DOX.

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    Effects of exercise on exercise capacity, cardiopulmonary function and cognitive function in elderly coronary heart disease patients with percutaneous coronary intervention
    ZHAO Mengfei, MAO Liwei, JI Peng, HU Shugang, GAO Miao, WANG Lei
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 198-206.   DOI: 10.12066/j.issn.1007-2861.2001
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    This paper aims to explore the comprehensive effect of exercise-based cardiac rehabilitation on the exercise capacity, cardiopulmonary function and cognitive function in elderly coronary heart disease (CHD) patients who have undergone percutaneous coronary intervention (PCI). The functional changes of 7 patients with PCI are observed before and after 12 weeks of exercise training. It is learned that the exercise capacity, cardiopulmonary function and cognitive function of the 7 patients are significantly improved after exercise training. The results indicate that exercise-based cardiac rehabilitation has positive effect on elderly CHD patients who have received PCI, and that it is conductive to improve the quality of life and reduce morbidity and mortality.

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    Photoelectric-response enhancement of local surface plasmon in Ge
    QI Gongmin, DI Zengfeng, REN Wei
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 207-216.   DOI: 10.12066/j.issn.1007-2861.1816
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    Local surface plasmon resonance (LSPR) is attracting much attention in nano-optoelectronics because of the unique photo response. Ge is an essential microelectronic material. But its photoelectric response is weak in the near-infrared region. Combination of the local surface plasmon and Ge can improve photoelectric response of Ge. The local surface plasmon resonance properties in Ge consisting of one, two and three silver nanoparticles cluster embedded in the Ge bulk are investigated using a finite difference time domain (FDTD) method. The extinction cross sections of one, two, and three silver nanoparticles are discussed in detail. The results show that the composite structure can effectively enhance extinction of Ge in a wide-range from visible to near-infrared. Moreover, the clusters show new types of photo responses as compared with single silver nanoparticle. The results also suggest that photo responses determined by local surface plasmon resonance depend strongly on conditions such as polarization directions of incident light, number of particles, size of single particle, and gap distances. This study is of significance for Ge applications and utilization of local surface plasmon resonance in optoelectronics.

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    Modeling and simulation of pedestrians evacuating from a classroom with two exits
    DONG Liyun, LAN Dongkai, DUAN Xiaoyin
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 217-224.   DOI: 10.12066/j.issn.1007-2861.1796
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    A cellular automaton model based on a time-varying floor field is applied to investigate crowd evacuation from a classroom with two exits. In this model, static obstacles are classified into impassable ones and passable ones. As pedestrians are treated as movable obstacles, the floor field varies with the crowd movement. Each exit produces its floor field, and a pedestrian selects his/her target exit by comparing values of both fields in his/her position. Numerical simulations are performed to study the evacuation process in consideration of exit width and desk layouts. It is shown that the key factor of exit choice is the distance to each exit in the evacuation process. Initially students prefer to enter the nearest aisle. In an evacuation process, few students in the middle of the classroom turn to another exit due to the congestion near the previously selected exit. Once they are approaching their target exits, they usually do not change their choices again. It coincides with the daily experience. This model can be used to simulate crowd evacuation from a multi-exit room with complex inner structures.

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    PMA:MoO$_3$ nanocomposite hole transport layer for organic solar cells
    WANG Yiling, YI Jinduo, LUO Qun, XIE Zhongming, LI Yanqing, MA Changqi, LUO Liqiang
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 225-235.   DOI: 10.12066/j.issn.1007-2861.1804
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    To develop PEDOT:PSS (poly (3,4-ethylendioxythiophene):poly (sodium-p-styrenesulfonate)—hole transporting materials in polymer solar cells, a solution-processable nanocomposite ink based on MoO$_3$ nano-particles and phosphomolybdic acid (PMA) is reported. The PMA:MoO$_3$ composite ink can be easily prepared by simply mixing PMA and MoO$_3$ solutions in different weight ratios. This PMA:MoO$_3$ composite ink shows good wettability on polymer surface. A smooth and homogeneous thin film can be easily deposited on polymer surface via a spin-coating process without any surface treatment. Both open circuit voltage ($V_ {OC}$) and fill factor (FF) of the PMA:MoO$_3$-based P3HT:PC$_{61}$BM cells are higher than that based on PMA or MoO$_3$ cells. Influence of the blend ratio between PMA and MoO3 on solar cell performance was carried out, and the optimized best blend ratio was found to be 1:2 for PMA:MoO$_3$, with which a highest device performance of 3.71% was achieved. The current work demonstrates that nanocomposite of metal oxide and polyoxometalate (POM) can serve as an excellent electrode buffer layer for solution-processed organic electronics.

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    Pressure fluctuation and vibration performance of centrifugal pump with gap drainage impeller
    ZHANG Wenzhu, WEI Qun, CHEN Hongxun, MA Zheng, WANG Daifeng
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 236-248.   DOI: 10.12066/j.issn.1007-2861.1827
    Abstract1195)   HTML17)    PDF(pc) (36474KB)(268)       Save

    Based on the theory of flow control, a gap drainage impeller structure is proposed. Compared with the ordinary impellers, the updated gap drainage impeller can improve hydraulic performance and cavitation performance of centrifugal pump in certain aspects. Experimental results show that, with increase of flow, pressure pulsation and vibration in the volute and outlet region decrease first, and then increase when flow becomes larger. With a large flow, pressure pulsation and vibration of the gap drainage impeller centrifugal pump is obviously weaker than those of the ordinary centrifugal pumps. Numerical investigation shows that the gap drainage impeller has more uniform velocity distribution on the outlet of channel and weaker inner secondary transportation. These are the main reasons for making the pressure pulsation of the gap drainage impeller centrifugal pump weaker. As pressure pulsation is one of the main causes of vibration, the gap drainage impeller can improve operation stability of a low specific centrifugal pump. The main frequency for the ordinary centrifugal pump is the blade passing frequency (BPF). In contrast, the main frequency for the gap drainage impeller centrifugal pump is double BPF, which is the result of a unique gap structure. The main frequency of vibration in horizontal and vertical directions is double BPF. As to mutual correlation of pressure pulsation and vibration, the amplitude of BPF and double BPF is higher, confirming that pressure pulsation and vibration are closely related, and excited by the rotation of impeller.

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    Numerical simulation on characteristics of levitated oscillating liquid metal drop in high frequency amplitude-modulated electromagnetic field
    ZHU Hongda, LEI Zuosheng, GUO Jiahong
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 249-256.   DOI: 10.12066/j.issn.1007-2861.1815
    Abstract723)   HTML12)    PDF(pc) (2804KB)(203)       Save

    Based on the experiment results of oscillation of a levitated liquid metal drop in a high frequency amplitude-modulated electromagnetic field, a two-dimensional axisymmetric numerical model for the flow of the drop is developed. The arbitrary Lagrangian-Eulerian (ALE) method is used to calculate free surface of the oscillating drop. The magnetic field, the flow field and the shape of the free surface of the droplet oscillating in a high frequency amplitude-modulated electromagnetic field are obtained by numerical simulation. By analyzing displacement of the characteristic point on the oscillating droplet over time using Fourier analysis, frequency spectrum characteristics of the drop in a high frequency amplitude-modulated electromagnetic field is obtained. Numerical results agree fairly well with the experimental and theoretical results.

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    Sharp estimates for Hardy operator with power weight on Heisenberg group
    CHEN Guoji, DONG Jianfeng
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 257-264.   DOI: 10.12066/j.issn.1007-2861.1817
    Abstract803)   HTML9)    PDF(pc) (484KB)(223)       Save

    In this paper, the $n$-dimensional Hardy operator with power weight on the Heisenberg group $H^n$ is studied. It is proved that the Hardy operator is a strong type of ($p, p$) ($1<p\leqslant \infty$) and a weak type of (1,1) on $L^p$($H^n$, $|x|_h^a$d$x$) and $L^1$ ($H^n$, $|x|_h^a$d$x$), respectively. Moreover, the results show that such ($p, p$) estimate is sharp, and obtain the upper and the lower bounds of the best constant of weak (1,1) type.

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    Solution and solid state properties of copper complex with aiminodiacetic derivative
    LIANG Qixian, GONG Teng, ZHAO Yongmei, ZHU Shourong
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 265-271.   DOI: 10.12066/j.issn.1007-2861.1776
    Abstract847)   HTML13)    PDF(pc) (4512KB)(482)       Save

    Reaction of the N,N,N',N'-p-xylylenediamine tetracetic acid (H$_4$L) with Cu(NO$_3$)$_2$ leads to the formation of a new polynuclear copper complex. Crystal structure of the polynuclear copper complex has been determined. This polynuclear copper complex belongs to the orthorhombic space group Pmn21, and exhibits a novel 3D structure. Rectangular channels with a dimension of 1.105 6 nm$\times$0.696 4 nm, based on the distances of Cu ions, are found in this structure. Additionally, the results of electro spray ionization-mass spectrometry (ESI-MS) spectra and potentiometric measurements show that the polynuclear copper complex exists as a dinuclear species in the solution. The results may provide theoretical basis to potential applications of the polynuclear copper complex in magnetism, catalysis and structural and functional models of metalloenzymes.

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    Influence of molding process parameters on compressive strength for polyimide resin and establishment of mathematical model
    FANG Lin, BURYA A I, YU Mingming, REN Musu, KALINICHENKO C B, EREMINA E A
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 272-277.   DOI: 10.12066/j.issn.1007-2861.1823
    Abstract1109)   HTML17)    PDF(pc) (760KB)(304)       Save

    Influence of molding temperature and duration time on the strength of polyimide were investigated, and a mathematical model was established. The results indicate that the pressing parameters have significant effects on the strength of polyimide. The compressive strength reaches maximum when the molding temperature is 653 K and non-pressure duration time is 25 min. Besides, a fitted quadric multiple equation is obtained with orthogonal central composite design. It can fully characterize compressive strength of polyimide within the research domain.

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    Effects of initial water content and sand content on dredger fill settlement
    WU Yajun, WEI Xing, TANG Jing
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 278-286.   DOI: 10.12066/j.issn.1007-2861.1798
    Abstract1108)   HTML16)    PDF(pc) (5098KB)(130)       Save

    Dredger fill settlement is a nonlinear process. The initial water content and sand content have direct effects on it. This research is carried out by settlement experiments on gravity settling characteristics. The effects of initial water content and sand content are analyzed. The settlement process is divided into three phases in which dredger fill's amount of settlement, settlement rate, void ratio and water content are analyzed. The results show that dredger fill with higher initial water content has more settling velocity, and settlement retard point appears earlier. After a settlement retard point appears, the amount of settlement becomes fewer. With lower initial water content, self-weight consolidation begins earlier, and the settlement retard point appears later. Sand content has less effect on the earlier settlement process, but has more effects on settlement in the consolidation stage. Sand content has little effects on dredger fill with high initial water content.

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    Stability analysis of geocell reinforced embankment with strength reduction method
    LIU Beibei, ZHANG Mengxi, WANG Dong
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 287-295.   DOI: 10.12066/j.issn.1007-2861.1801
    Abstract1035)   HTML12)    PDF(pc) (10712KB)(170)       Save

    It is difficult to see the contribution of changes in reinforcement parameters to a project with existing methods that analyze reinforced composite embankment. This paper uses a finite element software combined in conjunction with the strength reduction method (SRM) in numerical calculation to understand the effect of reinforcement of geocell. Calculations of 3D structural models for different reinforced embankments are carried out based on SRM. The results conform to that obtained by using limit equilibrium, and indicate feasibility of the proposed method for analyzing embankments under complex working conditions of the reinforcement. Stability of embankments with geocells is analyzed using the method. Influences of welding distance, buried depth, height and number of layers are then investigated. The results show that the geocell can increase global stability of the embankment. Also, effect of reinforcement can be improved by decreasing welding space and increasing height of the geocell. A fracture surface with several separate arcs will appear in the reinforced embankment with multi-layer geocells.

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    Soil pressure induced by pile driving based on model test
    ZHANG Yuchao, LU Ye
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 296-303.   DOI: 10.12066/j.issn.1007-2861.1777
    Abstract786)   HTML8)    PDF(pc) (10284KB)(190)       Save

    The paper presents laboratory measurements of soil radial stress obtained in two parallel pile driving tests. The tests were designed to impose two modes of load to investigate the underlying mechanism of the radial stress buildup. Analyses on the test results indicated that soil pressure reached a peak before the pile's tip reached the depth of the pressure sensor. The buildup of soil pressure was closely related to the penetration depth of the pile. The soil pressure measured by the pressure sensors increased slowly and then dissipated during the pile installation process. Peak radial stress buildup in the vicinity of the pile tip remained higher than that in other places. In general, the peak radial stress reduced quickly in response to dynamic loading compared with the results obtained from press-in loading.

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    Method of bond-slip model of near-surface mounted FRP strips-concrete interfaces
    ZHANG Zhimei, ZHANG Zhenkai, XIONG Hao, WANG Zhuo, CHEN Gang
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 304-313.   DOI: 10.12066/j.issn.1007-2861.1775
    Abstract775)   HTML13)    PDF(pc) (4118KB)(186)       Save

    A high-precision numerical method is proposed to study a bond-slip model for near-surface mounted fiber reinforced polymer (FRP) strips-concrete interfaces. With this method, undetermined parameters of a given bond-slip model can be determined only using the test data of every FRP steps' load and slips of the free end and loaded end in the related tests. A computer program is developed for the purpose. Reliability of the method is verified by a series of test date of pullout-bending bonded joints. Influences of bond length of FRP strips and strength of concrete on bond-slip model are analyzed. Crack problems of the near-surface mounted FRP concrete beam are studied based on the obtained bond-slip model of the interface.

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    Pencil drawing production based on hybrid gradient minimization
    WANG Quan, HU Yueli, YAN Ming, HU Yunsheng, CHEN Peng
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 314-321.   DOI: 10.12066/j.issn.1007-2861.1780
    Abstract800)   HTML13)    PDF(pc) (17091KB)(225)       Save

    Algorithms for creating pencil drawing effect from an image have two major problems: details are often missing so that important edge lines are not clearly shown, and lines are monotonous without hierarchical information. To solve these problems, this paper proposes a pencil drawing generation algorithm based on hybrid gradient minimization. By combining $L_0$ gradient minimization and $L_1$ norm, prominent edges are maintained and enhanced without affecting the overall sharpness. This way, rich and hierarchical details in the image are fully retained. Experimental results indicate that the proposed method can generate high quality and more realistic pencil drawing effect. It is superior to several other existing method.

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    System optimization for tomato Micro-Tom transgenic regeneration
    QIU Shi, ZHANG Wenju, XU Fuhui, DENG Zhirui
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (2): 322-330.   DOI: 10.12066/j.issn.1007-2861.1773
    Abstract1518)   HTML26)    PDF(pc) (5970KB)(297)       Save

    Low redifferentiation frequency of transgenic plant is a major barrier in plant gene engineering. Tomato species Micro-Tom was used as the experimental material. Effects of plant hormone combination, preculture time, and co-culture time of kanamycin and timentin on callusogenesis and adventitious bud growth were explored. The results show that cotyledon is more suitable than hypocotyledon as explants for callusogenesis and adventitous bud induction. Combination of 0.5 mg/L zeatin (ZT) with 1.0 mg/L indole acetic acid (IAA) brings about the highest adventitous bud induction. Suitable preculture time for explants is 3 d, and that for co-culture with Agrobacterium tumefaciens is 2 d. The upper tolerance limit of explant to kanamycin for selection is 40 mg/L and suitable timentin concentration for Agrobacterium tumefaciens inhibition is 150 mg/L. The established system may provide a good base for genetic engineering for Micro-Tom tomato.

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    Interpretation of 2017 American Guideline for Hypertension in Adults
    PAN Xin, XU Yawei, XU Dachun
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (3): 331-335.   DOI: 10.12066/j.issn.1007-2861.2003
    Abstract1007)   HTML177)    PDF(pc) (799KB)(464)       Save

    On November 14, 2017, American College of Cardiology (ACC) and American Heart Association (AHA), together with 9 other authorities, released 2017 American Guideline for Hypertension in Adults (2017ACC/AHA). The guideline no longer used the past diagnostic standard of hypertension, and it set up a new antihypertensive target. Ever since the issue of the guideline, experts and researchers around the world have been debating over the pros and cons of the new changes the guideline contains, and it can be expected that this guideline will continue to remain a controversial topic. This paper is an attempt to interpret the American guideline by taking into account the present situation in China.

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    Interpretation of Portal Hypertensive Bleeding in Cirrhosis: Risk Stratification, Diagnosis and Management: 2016 Practice Guidance by the American Association for the Study of Liver Diseases
    SONG Weiping, YANG Changqing
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (3): 336-340.   DOI: 10.12066/j.issn.1007-2861.2002
    Abstract853)   HTML12)    PDF(pc) (587KB)(139)       Save

    Hemorrhage resulting from high pressure of portal vein is a common complication of decompensated liver cirrhosis. Because of different courses, anatomic locations of varicose vein and complications, patients with cirrhosis need to be prudent in their choice of methods for diagnosis, treatment and prevention of rebleeding. Portal Hypertensive Bleeding in Cirrhosis: Risk Stratification, Diagnosis and Management: 2016 Practice Guidance by the American Association for the Study of Liver Diseases, which is based on a great deal of clinical data, may serve as a guideline for clinical practice in China as well. This paper, through close reading of the practice guidance, and through comparative analysis between the American guidance and that in China, hopes to shed some new light for medical practitioners on the handling of various problems connected with liver diseases.

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    Novel model of pedestrian detection based on Gaussian mixture model and HOG+SVM
    GONG Luming, XU Meihua, LIU Dongjun, ZHANG Fayu
    Journal of Shanghai University(Natural Science Edition)    2018, 24 (3): 341-351.   DOI: 10.12066/j.issn.1007-2861.1849
    Abstract1483)   HTML12)    PDF(pc) (15876KB)(340)       Save

    To improve detection rate of pedestrian detection system, a novel pedestrian detection model by combining the Gaussian mixture background model and histograms of oriented gradients (HOG) plus support vector machine (SVM) is proposed. First, foreground segmentation is done using the Gaussian mixture model (GMM) to extract moving target areas. In the recognition of pedestrians, the dimension of the HOG descriptor is reduced by resizing the size of the detecting window. In addition, an error recognition region is used to re-update the information of sample dataset to optimize the SVM classifier. Performance of this model is evaluated with the test frames randomly chosen from a video taken in a realistic scene. The results indicate that GMM and HOG+SVM can ensure accuracy and speed of detection, and limit false detection rate to 4 %. Real-time and accurate pedestrian detection in complex scenes are achieved.

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