Micro-Tom番茄转基因植株再生体系的优化

doi:10.12066/j.issn.1007-2861.1773

Abstract

Abstract:

Low redifferentiation frequency of transgenic plant is a major barrier in plant gene engineering. Tomato species Micro-Tom was used as the experimental material. Effects of plant hormone combination, preculture time, and co-culture time of kanamycin and timentin on callusogenesis and adventitious bud growth were explored. The results show that cotyledon is more suitable than hypocotyledon as explants for callusogenesis and adventitous bud induction. Combination of 0.5 mg/L zeatin (ZT) with 1.0 mg/L indole acetic acid (IAA) brings about the highest adventitous bud induction. Suitable preculture time for explants is 3 d, and that for co-culture with Agrobacterium tumefaciens is 2 d. The upper tolerance limit of explant to kanamycin for selection is 40 mg/L and suitable timentin concentration for Agrobacterium tumefaciens inhibition is 150 mg/L. The established system may provide a good base for genetic engineering for Micro-Tom tomato.

Key words: Micro-Tom tomato, zeatin (ZT), timentin, genetic transformation, Agrobacterium tumefaciens

CLC Number:

S641.2

QIU Shi, ZHANG Wenju, XU Fuhui, DENG Zhirui. System optimization for tomato Micro-Tom transgenic regeneration[J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(2): 322-330.

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System optimization for tomato Micro-Tom transgenic regeneration

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