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Journal of Shanghai University（Natural Science Edition）

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Characterization of Interaction between Salvianolic Acid B and Phages Using Capillary Electrophoresis

LIU Shu-peng1,3, SUN Ye1, GUO Wei2, XU Yu-hong2   

  1. 1. College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; 
    2. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; 
    3. School of Communication and Information Engineering, Shanghai University, Shanghai 200072, China
  • Received:2006-11-24 Revised:1900-01-01 Online:2008-02-28 Published:2008-02-28
  • Contact: XU Yu-hong

Cited

2

Abstract: Binding activity between Salvianolic acid B and selected phages from affinity biopanning in phage display library were studied with capillary lectrophoresis based on the Hummel-Dreyer methods. The binding constant was calculated with Scatchard analysis using PRISM software. The results indicate that the surface proteins expressed by the phage clone interact specifically with Salvianolic acid B, and it is feasible to determine the binding constant between the selected phage and Salvianolic acid B using CE.

Key words: capillary electrophoresis, phage, Salvianolic acid B

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