中文

Journal of Shanghai University（Natural Science Edition） ›› 2016, Vol. 22 ›› Issue (3): 336-343.doi: 10.3969/j.issn.1007-2861.2016.03.011

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Macrophage migration inhibitory factor deficiency aggravates cardiac hypertrophy induced by phenylephrine in mice

XIAO Zhen, ZHU Jiening, TANG Chunmei, LIN Qiuxiong, HU Zhiqin, ZHANG Zhuo, FU Yongheng, ZHANG Mengzhen, SHAN Zhixin   

  1. Research Department of Medical Science, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou 510080, China
  • Received:2016-04-19 Online:2016-06-30 Published:2016-06-30

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Abstract:

To investigate the effect of macrophage migration inhibitory factor (MIF) deficiency on the cardiac hypertrophy induced by hypodermic injection of phenylephrine (PE) in mice. A mouse model of cardiac hypertrophy induced by hypodermic injection of PE was established based on MIF-knockout (MIF-KO) mouse and the wide type control (WT) mouse. The left ventricular (LV) structure and function variables were assessed by transthoracic echocardiography. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay was performed to detect cardiomyocyte apoptosis. Expressions of SOD1, SOD2 and Trx2 were determined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western Blot assay, respectively. ①A mouse model of cardiac hypertrophy was achieved, induced by hypodermic injection of 20 mg/(kg·d) PE for 3 d. Compared with WT mice, PE injection induced more severe cardiac hypertrophy in MIF-KO mice. ②TUNEL assay revealed that the level of PE injection-induced cardiomyocte apoptosis in the myocardium of MIF-KO mouse was higher than that in WT mice. ③Expressions of SOD1 and Trx2 were significantly decreased in the myocardium of MIF-KO mice after PE injection, and reduction of Trx2 protein in myocardium of MIF-KO mice was more than that in WT mouse. MIF deficiency attenuates the expressions of SOD1 and Trx2, contributing to the aggravation of cardiomyocyte apoptosis and cardiac hypertrophy induced by hypodermic injection of PE in mice.

Key words:  cardiac hypertrophy,  cardiomyocyte ,  phenylephrine , macrophage migration inhibitory factor (MIF)