不同运动强度对急性心肌梗死大鼠心功能的影响及循环miRNAs差异表达

doi:10.3969/j.issn.1007-2861.2016.03.010

Abstract

Abstract:

To study the effects of different exercise intensity on cardiac function and differential expression of circulating microRNAs (miRNAs), target genes and gene function in rats with acute myocardial infarction (AMI). Establish AMI models with 40 rats, divide them into 4 groups, such as Sham operation group, isolated myocardial infarction group, continuous moderately training (CMT) group, and high intensity interval training (HIT) group, with 10 AMI rats in each group. CMT and HIT groups received exercise therapies for 8 weeks. Evaluate cardiac function with echocardiography. Analyze differential expression of circulating microRNAs, related target genes and gene function using miRNAs microarray and bioinformatics technology. CMT and HIT therapies significantly improved cardiac function and exercise tolerance in AMI rats. The effect of HIT group is significantly better than that of CMT group. Compared with Sham operation group, 14 circulating miRNAs were obviously up-regulated, 4 circulating miRNAs were obviously down-regulated in isolated myocardial infarction group. Compared with isolated myocardial infarction group, 11 circulating miRNAs were obviously up-regulated, 2 circulating miRNAs were obviously down-regulated in CMT group, and 53 circulating miRNAs were obviously up-regulated, 41 key miRNAs were obviously down-regulated in HIT group. Compared with Sham operation group, differential expression of myocardium related circulating miRNAs in isolated myocardial infarction group are miR-26a-5p, miR-92a-3p and miR-378a-3p. Compared with isolated myocardial infarction group, there is miR-92a-3p in CMT group, and there are miR-34c-3p, miR-23a-3p, miR-98-3p, miR-208a-5p and miR-92-3p in HIT group. HIT improving cardiac function and exercise tolerance in AMI rats are better than those of CMT. Numbers of differential expression of circulating miRNAs and myocardium related circulating miRNAs with HIT are higher than those of CMT, indicating that circulating miRNAs are expected to be biomarkers for determining exercise intensity and exercise effect.

Key words: differential expression, circulating microRNAs , exercise intensity , acute myocardial infarction

XIA Kun1, DING Rongjing2, LU Kai3, WANG Li4. Effects of exercise on cardiac function and differential expression of circulating miRNAs in rats with acute myocardial infarction[J]. Journal of Shanghai University（Natural Science Edition）, 2016, 22(3): 344-356.

References

[1] Oldridge N. Exercise-based cardiac rehabilitation in patients with coronary heart disease: Meta-analysis outcomes revisited [J]. Future Cardiol, 2012, 8: 729-751.
[2] Moholdt T, Aamot I L, Granøien I, et al. Aerobic interval training increases peak oxygen uptake more than usual care exercise training in myocardial infarction patients: a randomized
controlled study [J]. Clin Rehabil, 2012, 26: 33-44.
[3] Moholdt T, Aamot I L, Granøien I, et al. Long-term follow-up after cardiac rehabilitation: a randomized study of usual care exercise training versus aerobic interval training after myocardial infarction [J]. Int J Cardiol, 2011, 152: 388-390.
[4] Maiorana A. Interval training confers greater gains than continuous training in people with heart failure [J]. J Physiother, 2012, 58: 199.
[5] Wang G K, Zhu J Q, Zhang J T, et al. Circulating microRNA: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans [J]. Eur Heart J, 2010, 31(6): 659-666.
[6] Corsten M F, Dennert R, Jochems S, et al. Circulating microRNA-208b and microRNA-499 reflect myocardial damage in cardiovascular disease [J]. Circ Cardiovasc Genet, 2010, 3(6): 499-506.
[7] Adachi T, Nakanishi M, Otsuka Y, et al. Plasma microRNA-499 as a biomarker of acute myocardial infarction [J]. Clin Chem, 2010, 56(7): 1183-1185.
[8] Ma Z, Qi J, Meng S, et al. Swimming exercise training-induced left ventricular hypertrophy involves microRNAs and synergistic regulation of the PI3K/AKT/mTOR signaling pathway [J]. Eur J Appl Pysiciol, 2013, 113: 2473-2486.
[9] Martinelli N C, Cohen C R, Santos K G, et al. An analysis of the gobal expression of microRNAs in an experimental model of physiological left ventricular hypertrophy [J]. PLoS
One, 2014, 9: e93271.
[10] Høydal M A, Wisløff U, Kemi O J, et al. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training [J]. Eur J Cardiovasc Prev Rehabil, 2007, 14: 753-760.

[11] Kraljevic J, Marinovic J, Pravdic D, et al. Aerobic interval training attenuates remodelling and mitochondrial dysfunction in the post-infarction failing rat heart [J]. Cardiovasc Res, 2013, 99: 55-64.
[12] Moreira J B, Bechara L R, Bozi L H, et al. High-versus moderate-intensity aerobic exercise training effects on skeletal muscle of infarcted rats [J]. J Appl Physiol, 2013, 114: 1029-1041.
[13] Fernandes T, Bara?na V G, Negrão E, et al. Aerobic exercise training promotes physiological remodeling a set of microRNAs [J]. Am J Physiol Heart Circ Physiol, 2015, 309: 543-552.
[14] Seeger F H, Zeiher A M, Dimmeler S. MicroRNA in stem cell function and regenerative therapy of the heart [J]. Arterioscler Thromb Vasc Biol, 2013, 33: 1739-1746.
[15] Boon R A, Dimmeler S. MicroRNA in myocardial infarction [J]. Natrure Review Cardiology, 2015, 12: 135-142.
[16] Laterza O F, Lim L, Garrett-Engele P W, et al. Plasma microRNAs as sensitive and specific biomarkers of tissue injury [J]. Clin Chem, 2009, 55(11): 1977-1983.
[17] Mitchell P S, Parkin R K, Kroh E M, et al. Circulating microRNAs as stable blood-based markers for cancer detection [J]. Proc Natl Acad Sci USA, 2008, 105(30): 10513-10518.
[18] Hunter M P, Ismail N, Zhang X, et al. Detection of microRNAs expression in human peripheral blood microvesicles [J]. PLoS One, 2008, 3(11): e3694.
[19] Timmons J A, Jansson E, Fischer H, et al. Modulation of extracellular matrix genes reflects the magnitude of physiological adaptation to aerobic exercise training in humans [J]. BMC Biol, 2005, 3: 19.
[20] Keller P, Vollaard N, Babraj J, et al. Using systems biology to define the essential biological networks responsible for adaptation to endurance exercise training [J]. Biochem Soc Trans, 2007, 35(Pt5): 1306-1309.
[21] Leon A S, Franklin B A, Costa F, et al. Cardiac rehabilitation and secondary prevention of coronary heart disease: an American Heart Association scientific statement from the Council on Clinical Cardiology (Subcommittee on Exercise, Cardiac Rehabilitation, and Prevention) and the Council on Nutrition, Physical Activity, and Metabolism (Subcommittee on Physical Activity), in collaboration with the American Association of Cardiovascular and Pulmonary Rehabilitation [J]. Circulation, 2005, 111(3): 369-376.
[22] Corrà U, Piepoli M F, Carr´e F, et al. Secondary prevention through cardiac rehabilitation: physical activity counselling and exercise training: key components of the position paper from the Cardiac Rehabilitation Section of the European Association of Cardiovascular Prevention and Rehabilitation [J]. Eur Heart J, 2010, 31(16): 1967-1974.
[23] 中华医学会心血管病学分会, 中国康复医学会心血管病专业委员会, 中国老年学学会心脑血管病专业委员会. 冠心病心脏康复与二级预防中国专家共识[J]. 中华心血管病杂志, 2013, 41(4): 267-275.
[24] Golbidi S, Laher I. Exercise and the cardiovascular system [J]. Cardiol Res Pract, 2012, 2012:201-206.

Effects of exercise on cardiac function and differential expression of circulating miRNAs in rats with acute myocardial infarction

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