大气颗粒物对A549 和HUVEC 的DNA 损伤机制

doi:10.3969/j.issn.1007-2861.2013.04.015

Abstract

Abstract: Ambient particulate matter has become one of the most harmful pollutants in the air. The genotoxic effects and generationmechanism of reactive oxygen species (ROS) were studied in A549 and HUVEC cells treated with three sizes of ambient particles, PM_10−2.5, PM_2.5−0.1and PM_0.1. The results from comet assay show that PM_10−2.5, PM_2.5−0.1 and PM_0.1 caused significant DNA damages in both A549 and HUVEC cells in a dose-dependent manner. In both types of cells, the DNA damage caused by PM_2.5−0.1 and PM_0.1are significantly higher than that caused by PM_10−2.5, and PMs cause higher DNA damage in HUVEC cells than that in A549 cells. PM_2.5−0.1 and PM_0.1 can significantly increase the intracellular ROS level, but PM_10−2.5cannot. DNA damage in cells induced by particles may be related to the generation of ROS, which deserves further investigations.

Key words: ambient particulate matter, DNA damage, human lung epithelial cell (A549), human umbilical vein endothelial cell (HUVEC), ROS generation

CLC Number:

R 994.6

SHANG Yu, ZHANG Ling, FAN Lan-lan, JIANG Yu-ting. Genotoxicity of Size-Fractionated Ambient Particulate Matter in A549 and HUVEC Cell Lines[J]. Journal of Shanghai University（Natural Science Edition）, 2013, 19(4): 411-415.

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Genotoxicity of Size-Fractionated Ambient Particulate Matter in A549 and HUVEC Cell Lines

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