1-硝基芘和苯并[a] 芘对人肺上皮A549 细胞的联合细胞毒性

doi:10.3969/j.issn.1007-2861.2016.01.008

Journal of Shanghai University（Natural Science Edition） ›› 2016, Vol. 22 ›› Issue (2): 181-187.doi: 10.3969/j.issn.1007-2861.2016.01.008

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Combined effects of 1-nitropyrene and benzo(a)pyrene on cytotoxicity and DNA damage in human lung epithelial A549 cells

SHANG Yu, ZHOU Qian, JIANG Yuting

Received:2016-01-12 Online:2016-04-30 Published:2016-04-30

Abstract

Abstract:

Using human lung epithelial A549 cells, combined toxic effects of 1-nitropyrene (1-NP) and benzo(a)pyrene (B[a]p) were evaluated. The 1-NP caused a significantly concentration-dependent viability decrease, DNA damage and reactive oxygen species (ROS) generation. Compared with the groups treated with 1-NP alone, viability was significantly increased and ROS generation was significantly reduced in combined-treated groups with 1-NP and B[a]p. However, the DNA damage was significantly increased in the combinedtreated groups compared with the groups treated with 1-NP alone. These results suggested that 1-NP may mediate the cytotoxic effects through ROS generation, and pretreatment, with B[a]p may inhibit ROS generation induced by 1-NP, and thereby reducing the cell death in A549 cells. However mechanisms of DNA damage deserves further investigations.

Key words: benzo(a)pyrene (B[a]P) , DNA damage , particulate matter , reactive oxygen species (ROS), 1-nitropyrene (1-NP)

SHANG Yu, ZHOU Qian, JIANG Yuting. Combined effects of 1-nitropyrene and benzo(a)pyrene on cytotoxicity and DNA damage in human lung epithelial A549 cells[J]. Journal of Shanghai University（Natural Science Edition）, 2016, 22(2): 181-187.

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Combined effects of 1-nitropyrene and benzo(a)pyrene on cytotoxicity and DNA damage in human lung epithelial A549 cells

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