中文

Journal of Shanghai University（Natural Science Edition） ›› 2024, Vol. 30 ›› Issue (2): 208-217.doi: 10.12066/j.issn.1007-2861.2552

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Plant-derived extracellular vesicles loaded with ACE2 prevent cell damage after PM2.5 exposure

YE Xuan, QIAN Yuan, WANG Hongyun, ZHOU Lei   

  1. 1. Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; 2. School of Life Science, Shanghai University, Shanghai 200444, China
  • Online:2024-04-30 Published:2024-05-14

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Abstract: To investigate the efficacy of plant-derived extracellular vesicles (PDEVs) loaded with angiotensin-converting enzyme 2 (ACE2) in mitigating respiratory system dam-age induced by particulate matter (PM2.5), we extracted PDEVs from grapes and loaded them with ACE2 overexpression plasmids. The effect of the modified PDEVs on human bronchial epithelial cells (Beas-2B) after exposure to PM2.5 was investigated using CCK8 assays, dihydroethidium (DHE) staining, western blotting, and real-time quantitative poly-merase chain reaction (PCR). Exposure to PM2.5 increases oxidative stress, apoptosis, and inflammatory factor production in Beas-2B cells. PDEVs loaded with ACE2 effectively delivered ACE2 to cells and thereby alleviated PM2.5-induced cell damage including apop-tosis, oxidative stress, and inflammation. PDEVs thus show potential for use as carriers of genes that can prevent apoptosis and damage caused by atmospheric PM2.5 exposure.

Key words: extracellular vesicles, angiotensin-converting enzyme 2 (ACE2), PM2.5, grapes

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