PCR效率振荡与金纳米粒子浓度的光量子机理

doi:10.12066/j.issn.1007-2861.2590

摘要/Abstract

摘要： 纳米材料在聚合酶链式反应(polymerase chain reaction,PCR)技术中的广泛应用为改进生物医学领域的检测方法开辟了新途径.已有实验揭示了PCR效率与pM区金纳米粒子浓度间的振荡行为,其产生或与带电胶体粒子间的长程库仑作用和纳米颗粒电子态的量子尺寸效应相关联.通过蒙特卡洛模拟可以发现,溶液中金纳米粒子的径向分布函数随着电荷的增加逐步呈现出峰值特征,从而引发光子在溶液中瑞利散射的相干行为,影响PCR反应过程中释放光子的再利用效率.研究结果表明,振荡周期与下游反应光子的波长吻合,同时其能量与金纳米粒子费米能级附近的能级宽度相匹配.而金纳米粒子可以吸收并储存于其内部的电子态,通过再释放促进PCR上游反应进程,并通过电子的玻尔兹曼分布来弥补所需能量的不足部分.该研究有望推动PCR特有的精确探测手段在量子生物科技领域的应用.

关键词: 金纳米粒子, 聚合酶链式反应, 长程库仑作用, 瑞利相干散射, 量子尺寸效应

Abstract: The widespread application of nanomaterials in polymerase chain reaction (PCR) technology has opened new avenues for improving detection methods in the biomedical field. Recent experiments have revealed that the oscillatory behavior between PCR efficiency and the concentration of gold nanoparticles in the pM range is, potentially linked to the long-range Coulomb interactions among charged colloidal particles and the quantum size effect of nanoparticles electronic states. Through Monte Carlo simulation, this paper discovered that the radial distribution function of gold nanoparticles in solution gradually exhibited peak characteristics with increasing charge, triggering coherent photon behavior in Rayleigh scattering within the solution, and thereby influencing the efficiency of reusing released photons in the PCR reaction. The study demonstrates that the oscillation period aligns with the wavelength of downstream reaction photons, while their energy matches the width of energy levels near the Fermi level of gold nanoparticles. The latter can absorb and store electron states internally, promoting upstream PCR reactions through subsequent re-release, and compensating for energy deficiencies through the Boltzmann distribution of electrons. This work is poised to advance the application of PCR-specific precise detection methods in the field of quantum biotechnology.

Key words: gold nanoparticles, polymerase chain reaction (PCR), long-range Coulomb interaction, Rayleigh coherent scattering, quantum size effect

中图分类号:

O469

方欢欢, 陈永聪. PCR效率振荡与金纳米粒子浓度的光量子机理[J]. 上海大学学报(自然科学版), 2025, 31(4): 646-656.

FANG Huanhuan, CHEN Yongcong. Light quantum mechanism of PCR efficiency oscillation concerning gold nanoparticle concentration[J]. Journal of Shanghai University（Natural Science Edition）, 2025, 31(4): 646-656.

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