1-氯-3-丁烯-2-醇对人胚肝 L02 细胞中内源性 H$_{\textbf{2}}$S 的抑制效应

doi:10.12066/j.issn.1007-2861.1825

上海大学学报(自然科学版) ›› 2018, Vol. 24 ›› Issue (5): 800-806.doi: 10.12066/j.issn.1007-2861.1825

1-氯-3-丁烯-2-醇对人胚肝 L02 细胞中内源性 H$_{\textbf{2}}$S 的抑制效应

张芳, 张新宇()

上海大学环境与化学工程学院环境污染与健康研究所, 上海 200444

收稿日期:2016-10-07 出版日期:2018-10-30 发布日期:2018-10-26
通讯作者: 张新宇 E-mail:xyzhang999@shu.edu.cn
基金资助:
国家自然科学基金资助项目(21077070);国家自然科学基金资助项目(21377080);上海市教委科研创新基金重点资助项目(11ZZ90)

Inhibition of endogenous H$_\textbf{2}$S concentrations in human hepatocyte L02 cells by 1-chloro-2-hydroxy-3-butene

ZHANG Fang, ZHANG Xinyu()

Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China

Received:2016-10-07 Online:2018-10-30 Published:2018-10-26
Contact: ZHANG Xinyu E-mail:xyzhang999@shu.edu.cn

摘要/Abstract

摘要：

1-氯-3-丁烯-2-醇(1-chloro-2-hydroxy-3-butene, CHB)是环境污染物1,3-丁二烯的一种代谢产物, 具有细胞毒性、遗传毒性和致突变性. H$_{2}$S是继 NO 和 CO 之后发现的第 3 种气体第二信使分子, 涉及多种生理功能, 其代谢异常与很多疾病存在密切联系. 研究了 CHB 对人胚肝 L02 细胞中内源性 H$_{2}$S 浓度的影响. 结果发现, CHB 可明显降低细胞内源性 H$_{2}$S 的浓度, 且降低程度取决于 CHB 浓度的高低. 因此, CHB 对内源性 H$_{2}$S 产生的抑制效应可能是其毒性的一种机理.

关键词: 1-氯-3-丁烯-2-醇, H$_{2}$S, 1,3-丁二烯, 毒性

Abstract:

1-chloro-2-hydroxy-3-butene (CHB) is a metabolite of 1,3-butadiene, an environmental pollutant. CHB is cytotoxic, genotoxic, and mutagenic. H$_2$S is the third gasotransmitter following NO and CO, and is involved in a variety of physiological activities. Abnormal metabolism of H$_2$S is associated with many diseases. In the present study, the effect of CHB on the concentrations of endogenous H$_2$S in human hepatocyte L02 cells was investigated. It was observed that CHB could cause significant decreases in the concentrations of endogenous H$_2$S in the cells and the effect depended on CHB concentration. Thus, inhibition of endogenous H$_2$S production in cells by CHB may be a mechanism for toxicity of CHB, which can contribute to a better understanding of toxicity of 1,3-butadiene.

Key words: 1-chloro-2-hydroxy-3-butene (CHB), H$_2$S, 1,3-butadiene, toxicity

中图分类号:

R994.6

张芳, 张新宇. 1-氯-3-丁烯-2-醇对人胚肝 L02 细胞中内源性 H$_{\textbf{2}}$S 的抑制效应[J]. 上海大学学报(自然科学版), 2018, 24(5): 800-806.

ZHANG Fang, ZHANG Xinyu. Inhibition of endogenous H$_\textbf{2}$S concentrations in human hepatocyte L02 cells by 1-chloro-2-hydroxy-3-butene[J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(5): 800-806.

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1-氯-3-丁烯-2-醇对人胚肝 L02 细胞中内源性 H$_{\textbf{2}}$S 的抑制效应

Inhibition of endogenous H$_\textbf{2}$S concentrations in human hepatocyte L02 cells by 1-chloro-2-hydroxy-3-butene

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