$O_{3}$氧化黑碳对SH-SY5Y细胞的毒性作用

doi:10.12066/j.issn.1007-2861.1963

上海大学学报(自然科学版) ›› 2019, Vol. 25 ›› Issue (4): 550-557.doi: 10.12066/j.issn.1007-2861.1963

$O_{3}$氧化黑碳对SH-SY5Y细胞的毒性作用

尚羽(), 王田田, 吴美英, 王璐, 何慧心, 安静

上海大学环境与化学工程学院环境污染与健康研究所, 上海 200444

收稿日期:2017-07-12 出版日期:2019-08-30 发布日期:2019-09-04
通讯作者: 尚羽 E-mail:yushang@shu.edu.cn
基金资助:
国家自然科学基金资助项目(91543123);国家自然科学青年基金资助项目(21107068);创新团队发展计划资助项目(IRT13078)

Cytotoxicity in SH-SY5Y cells induced by $O_{3}$-oxidized black carbon particles

Yu SHANG(), Tiantian WANG, Meiying WU, Lu WANG, Huixin HE, Jing AN

Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China

Received:2017-07-12 Online:2019-08-30 Published:2019-09-04
Contact: Yu SHANG E-mail:yushang@shu.edu.cn

摘要/Abstract

摘要：

以人神经母细胞瘤细胞SH-SY5Y为模型, 在细胞体系探讨经O$_{3}$氧化过的黑碳(O$_3$-oxidized black carbon, OBC)颗粒对神经细胞的毒性作用. 应用MTT方法评估SH-SY5Y细胞的存活率; 通过测定培养液中乳酸脱氢酶(lactate dehydrogenase, LHD)浓度评估细胞膜的损伤程度; 利用流式细胞术评价细胞凋亡率; 使用荧光探针(Fluo 3-AM)检测细胞内Ca$^{2+}$浓度. 结果显示, OBC颗粒对SH-SY5Y细胞暴露24和48 h的半致死浓度(IC$_{50})$分别为202.2和133.3 $\mu $g/mL; 随着BC颗粒染毒浓度的增加(0, 5, 10, 20, 40 $\mu $g/mL), 胞外LDH浓度、细胞凋亡率和Ca$^{2+}$浓度均逐渐升高, 呈显著的剂量-效应关系($p<$0.05). 研究表明, OBC颗粒可导致神经细胞内Ca$^{2+}$浓度升高、细胞膜损坏, 并最终导致细胞凋亡的发生.

关键词: 黑碳颗粒, SH-SY5Y细胞, 细胞毒性, 细胞凋亡, Ca$^{2+}$

Abstract:

SH-SY5Y cells were used to investigate neurotoxicity of O$_3$-oxidized black carbon (OBC) particles. Cell viability was measured by MTT assay after being treated with OBC for 24 and 48 h. Lactate dehydrogenase (LDH) leakage was determined to evaluate cellular membrane injury after OBC treatment for 24 h. Apoptosis rate was detected by cytometry after OBC treatment for 24 h. Concentrations of Ca$^{2+}$ was then measured with fluorescent probe of Fluo 3-AM. OBC exposure caused significant dose-dependent and time-dependent viability decrease. LC$_{50}$ for 24 and 48 h were 202.2 and 133.3 $\mu $g/mL, respectively. The LDH leakage, apoptosis rate and intracellular Ca$^{2+}$ levels were also increased significantly in a dose-dependent manner after being treated with OBC in SH-SY5Y cells. These results suggest that OBC may mediate cytotoxic effects and apoptosis through Ca$^{2+}$ release.

Key words: black carbon (BC) particles, SH-SY5Y cells, cytotoxicity, apoptosis, Ca$^{2+}$

中图分类号:

X513

尚羽, 王田田, 吴美英, 王璐, 何慧心, 安静. $O_{3}$氧化黑碳对SH-SY5Y细胞的毒性作用[J]. 上海大学学报(自然科学版), 2019, 25(4): 550-557.

Yu SHANG, Tiantian WANG, Meiying WU, Lu WANG, Huixin HE, Jing AN. Cytotoxicity in SH-SY5Y cells induced by $O_{3}$-oxidized black carbon particles[J]. Journal of Shanghai University（Natural Science Edition）, 2019, 25(4): 550-557.

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$O_{3}$氧化黑碳对SH-SY5Y细胞的毒性作用

Cytotoxicity in SH-SY5Y cells induced by $O_{3}$-oxidized black carbon particles

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