Abstract: To establish an RP-HPLC fingerprint analysis for quality control of Ganoderma lucidum, HPLC fingerprint analysis method of Ganoderma lucidum was developed. ZORBAX Eclipse XDB-C18 (4.6 mm×250 mm, 5-Micon) was used, with mixture of acetonitrile and water solution of acetic acid as mobile phase in a gradient mode. The flow rate was 0.8 mL/min. The wavelength used in the measurement was 242 nm. Ten batches of Ganoderma lucidum were determined. The HPLC fingerprint chromatograms of different producing area were analyzed. The samples’ quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004. The method can be used to identify and evaluate the quality of Ganoderma lucidum conveniently.

Key words: fingerprint, HPLC, quality control, Ganoderma lucidum