固相萃取-高效液相色谱检测吸烟人群尿中8 种单羟基多环芳烃

Abstract

Abstract: To assess the exposure to polycyclic aromatic hydrocarbons (PAHs) by smoking, a method to detect 8 types of urinary hydroxylated metabolites of PAHs (OH-PAHs) (containing 2 to 5 rings) was developed by using solid phase extraction (SPE）coupled to high performance liquid chromatography fluorescence detection (HPLC-FD). With optimized SPE and HPLC-FD conditions, 8 standard curve equations were established for individual OH-PAHs with linear ranges of 0.2~20 μg/L or 0.5~20 μg/L (all determination coefficients R²> 0.998). The method quantification limits (MDLs) were 0.03~0.07 μg/L, and recoveries R were (70%±4%)~(109%±30%), while inter-day variations ranged from 5% to 28%. OH-PAHs in urine samples from local smokers were successfully detected, showing that the method is promising in assessing exposure to PAHs for local smokers.

Key words: biomarker, health risk assessment, hydroxylated metabolite of polycyclic aromatic carbons, smoker, urine

CLC Number:

O 614.3

LI Yin-ping, LI Xue. Detection of Eight Hydroxylated Metabolites of Polycyclic Aromatic Hydrocarbons Smokers’ Urine Using Solid Phase Extraction Coupled to High Performance Liquid Chromatography[J]. Journal of Shanghai University（Natural Science Edition）, 2013, 19(4): 374-379.

References

[1] Vijayalakshmi K P, Suresh C H. Theoretical studies

on the carcinogenicity of polycyclic aromatic hydrocarbons

[J]. Journal of Computational Chemistry,

2008, 29: 1808-1817.

[2] 牛红云, 蔡亚岐, 魏复盛, 等. 多环芳烃暴露的生物标

志物——尿中羟基多环芳烃[J]. 化学进展, 2006, 18:

1381-1390.
[3] Campo L, Rossella F, Pavanello S, et al. Urinary

profiles to assess polycyclic aromatic hydrocarbons

exposure in coke-oven workers [J]. Toxicology

Letters, 2010, 192: 72-78.

[4] Kang R H, Wang Y S, Yang H M, et al.

Rapid simultaneous analysis of 1-hydroxypyrene,

2-hydroxyfluorene, 9-hydroxyphenanthrene, 1- and 2-

naphthol in urine by first derivative synchronous fluorescence

spectrometry using Tween-20 as a sensitizer

[J]. Analytica Chimica Acta, 2010, 658: 180-

186.

[5] 沈惠麒, 顾祖维, 吴宜群. 生物监测和生物标志物——

理论基础及应用[M]. 2 版. 北京: 北京大学医学出版

社, 2006: 3-17.

[6] Chetiyanukornkul T, Toriba A, Kizu R, et al.

Urinary 2-hydroxyfluorene and 1-hydroxypyrene levels

in smokers and nonsmokers in Japan and Thailand

[J]. Polycyclic Aromatic Compounds, 2003, 24:

467-474.

[7] Benowitz N L, Jacob P, Bernert J T, et al. Carcinogen

exposure during short-term switching from

regular to “Light” cigarettes [J]. Cancer Epidemiology

Biomarkers & Prevention, 2005, 14: 1376-1383.

[8] Peuss R, Drexler H, Bottcher M, et al. Current

external and internal exposure to naphthaleneof workers occupationally exposed to polycyclic aromatic

hydrocarbons in different industries [J]. International

Archives of Occupational and Environmental

Health, 2005, 78(5): 355-362.

[9] Jacob P, Wilson M, Benowitz N L. Determination

of phenolic metabolites of polycyclic

aromatic hydrocarbons in human urine as their

pentafluorobenzyl ether derivatives using liquid

chromatography-tandem mass spectrometry [J]. Analytical

Chemistry, 2007, 79(2): 587-598.

[10] Hagedorn H W, Scherer G, Engl J, et al. Urinary

excretion of phenolic polycyclic aromatic hydrocarbons

(OH-PAH) in nonsmokers and in smokers of

cigarettes with different ISO tar yields [J]. Journal of

Analytical Toxicology, 2009, 33(6): 301-309.

[11] Ramsauer B, Sterz K, Hagedorn H W, et al.

A liquid chromatography/tandem mass spectrometry

(LC-MS/MS) method for the determination of phenolic

polycyclic aromatic hydrocarbons (OH-PAH) in

urine of non-smokers and smokers [J]. Analytical and

Bioanalytical Chemistry, 2011, 39(2): 877-889.

[12] Sthelen G, Goniewicz M L, Demosey D, et al.

Exposure and kinetics of polycyclic aromatic hydrocarbons

(PAHs) in cigarette smokers [J]. Chemical

Research in Toxicology, 2012, 25(4): 952-964.

[13] Scherer G, Frank S, Riedel K, et al. Biomonitoring

of exposure to polycyclic aromatic hydrocarbons

of nonoccupationally exposed persons [J]. Cancer

Epidemiology Biomarkers & Prevention, 2000, 9:

373-380.

[14] Hecht S S, Carmella S G, Le K A, et al.

Effects of reduced cigarette smoking on levels of1-hydroxypyrene in urine [J]. Cancer Epidemiology

Biomarkers & Prevention, 2004, 13: 834-842.

[15] Lafontaine M, Champmartin C, Simon P, et al.

3-hydroxybenzo a pyrene in the urine of smokers and

non-smokers [J]. Toxicology Letters, 2006, 162: 181-

185.

[16] Chetiyanukornkul T, Toriba A, Kameda T, et

al. Simultaneous determination of urinary hydroxylated

metabolites of naphthalene, fluorene, phenanthrene,

fluoranthene and pyrene as multiple biomarkers

of exposure to polycyclic aromatic hydrocarbons

[J]. Analytical and Bioanalytical Chemistry,

2006, 386: 712-718.

[17] Elovaara E, Mikkola J, M¨akel¨a M, et al. Assessment

of soil remediation workers’ exposure to polycyclic

aromatic hydrocarbons (PAHs): biomonitoring

of naphthols, phenanthrols, and 1-hydroxypyrene in

urine [J]. Toxicology Letters, 2006, 162: 158-163.

[18] Buratti M, Campo L, Fustinoni S, et al. Urinary

hydroxylated metabolites of polycyclic aromatic

hydrocarbons as biomarkers of exposure in asphalt

workers [J]. Biomarkers, 2007, 12(3): 221-239.

[19] Kuusimaki L, Peltonen Y, Mutanen P, et al.

Urinary-metabolites of naphthalene, phenanthrene

and pyrene as biomarkers of exposure to diesel exhaust

[J]. International Archives of Occupational and

Environmental Health, 2004, 77: 23-30.

[20] Liu A L, Lu W Q, Wang Z Z, et al. Elevated levels

of urinary 8-hydroxy-2’-deoxyguanosine, lymphocytic

micronuclei, and serum glutathione s-transferase in

workers exposed to cokeoven emissions [J]. Environmental

Health Perspectives, 2006, 114: 673-677.

Detection of Eight Hydroxylated Metabolites of Polycyclic Aromatic Hydrocarbons Smokers’ Urine Using Solid Phase Extraction Coupled to High Performance Liquid Chromatography

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Recommended Articles

Metrics

Comments

[1]	LIU Boliang, ZHANG Xinyu. Reaction of 1-chloro-3-buten-2-one with N-acetylcysteine [J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(1): 74-82.
[2]	XU Dongxu, ZHANG Haifeng, LI Xinli. Interpretation of Role of biomarkers for the prevention, assessment, and management of heart failure [J]. Journal of Shanghai University（Natural Science Edition）, 2017, 23(6): 821-.
[3]	ZHANG Chunchun1,2, HE Chiquan1. PM_2.5 and its heavy metal contents in Shanghai and the assessment of health risk to residents [J]. Journal of Shanghai University（Natural Science Edition）, 2016, 22(6): 784-792.
[4]	WANG Fei, Saumya Das. Circulating miRNAs predict response to cardiac resynchronization therapy [J]. Journal of Shanghai University（Natural Science Edition）, 2016, 22(3): 265-269.
[5]	LOU Sufang1, WANG Xinxin1, JIANG Zi’an1, LU Shaoyou2, YU Yingxin1. Levels of polybrominated diphenyl ethers in surface soil of Guiyu and the associated health risk to children [J]. Journal of Shanghai University（Natural Science Edition）, 2016, 22(2): 141-150.