气相色谱/质谱法同时检测鱼肉样品中三氯生及其甲基代谢产物

doi:10.3969/j.issn.1007-2861.2013.04.005

Abstract

Abstract: Gas chromatography coupled with mass spectrometry (GC/MS) was developed for the simultaneous determination of triclosan (TCS) and methyl-triclosan (MTCS) in fish samples. Parameters affecting both derivatization and clean-up procedures were studied. After extracting with hexane and actone (Vhexane∶Vactone=1∶1), cleaning-up with gel permeation chromatography (GPC) and silica gel packed column, the residues were derivatized with acetic anhydride and analyzed by GC/MS. Under the optimal conditions, the linear range was 5~500 pg/μL for TCS and 2~500 pg/μL for MTCS. The correlation coefficient R was above 0.99 for the analytes, with the limit of detection (LOD) 1.3 and 0.8 pg/μL, respectively. Good recoveries (83%~89% for TCS and 85%~98% for MTCS) were achieved in the spiking experiments. The established method was successfully applied to determine this analytes in real fish samples.

Key words: gas chromatography/mass spectrometry (GC/MS);fish samples, methyl-triclosan (MTCS), triclosan (TCS)

CLC Number:

TU 411

References

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Simultaneous Analysis of Triclosan and Methyl-triclosan in Fish Samples by Gas Chromatography Coupled with Mass Spectrometry

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