Journal of Shanghai University(Natural Science Edition) ›› 2012, Vol. 18 ›› Issue (4): 430-434.doi: 10.3969/j.issn.1007-2861.2012.04.019
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DENG Zhi-rui,SUN Jing-jing,ZHANG Wen-ju,CHEN Qin
Online:
Published:
Abstract: This paper studies the effects of different extraction solution, ratio of sampleweight to extractionsolution and concentrations of reduction reagent dithiothreito (DTT) on extraction efficiency and quality of electrophoresis of prolamin from seeds of Sorghum sudanense. Contribution of the gel concentration and loading dosage to the results of SDSPAGE were analyzed by evaluating the electrophoresis. The results show that 70% propylalcohol supplemented with 0.2% DTT is the best extraction solution and the ratio of sampleweight to extractionsolution 1∶8 (g/mL) can give optimal results. SDSPAGE of 12% produces the best clearance for electrophoresis under a loading appropriate sample. Established condition based on prolamin from seeds of Sorghum sudanense can be applied to isolation and electrophoresis for prolamins from several other gramineae seeds with good results.
Key words: Sorghum sudanense, prolamin extraction, SDS-PAGE
CLC Number:
O 512+.5
DENG Zhi-rui,SUN Jing-jing,ZHANG Wen-ju,CHEN Qin. Optimization of Extraction and Electrophoresis of Prolamin from Sorghum sudanense seeds[J]. Journal of Shanghai University(Natural Science Edition), 2012, 18(4): 430-434.
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URL: https://www.journal.shu.edu.cn/EN/10.3969/j.issn.1007-2861.2012.04.019
https://www.journal.shu.edu.cn/EN/Y2012/V18/I4/430