柴油和镉污染对土壤酶活性和微生物数量的影响

doi:10.12066/j.issn.1007-2861.2233

Abstract

Abstract:

A pot experiment was conducted to study the effects of pollutants of diesel fuel and Cd alone or in combination on the activities of urease (UR) and dehydrogenase (DE) and microbial biomass in non-polluted paddy soil collected from an urban setting in Shanghai. The experiment included seven treatments: control (CK), low content of diesel fuel (CYL), high content of diesel fuel (CYH), low content of Cd (CdL), high content of Cd (CdH), combination of high content of diesel fuel and Cd (CYH+CdH), and combination of high content of diesel fuel, Cd and crop seedlings (CYH+CdH+P). The activity of UR was significantly raised when the soil was polluted with CYH and Cd for the treatments of CYH and CYH + CdH. The maximum UR for the two treatments was 5.25 and 2.63 times that of CK, respectively. The microbial biomass for the treatments was also correspondingly raised to some extent. Conversely, the activity of DE in the soil for the CYL, CYH and CYH + CdH treatments was significantly inhibited. The influence of Cd on DE activity in the soil was relatively mild. DE was activated even when the soil was polluted with CdL.

Key words: diesel fuel, Cd, soil, urease (UR), microbial biomass

CLC Number:

X825

HE Zichen, HU Xuefeng, LU Siwen, ZHAO Jinglong, LAN Guojun, LI Mei, ZHANG Weijie. Effects of diesel fuel and Cd pollution on enzyme activity and microbial biomass in the soil[J]. Journal of Shanghai University（Natural Science Edition）, 2022, 28(1): 31-39.

Figures/Tables 6

Table 1

Table 2

Table 3

Fig. 1

Fig. 2

Fig. 3

References 41

[1]	周礼恺, 郑巧英. 石油烃和酚类物质在土中的生物降解与土壤酶活性[J]. 应用生态学报, 1990, 1(2): 149-155.
[2]	杨勇, 刘馨璐, 张凤泉, 等. 国Ⅳ、国Ⅴ车用柴油烃类组成分析[J]. 石油商技, 2016, 34(6): 40-43.
[3]	张钊, 张海清. 湖南省镉污染土壤现状及建议[J]. 现代农业科技, 2019(10): 150-151, 153.
[4]	中华人民共和国生态环境部. 全国土壤污染状况调查公报[J]. 中国环保产业, 2014(5): 10-11.
[5]	安玉姿. 土壤石油污染的危害与修复[J]. 中国资源综合利用, 2017, 35(5): 72-73.
[6]	曾咏梅, 毛昆明, 李永梅, 等. 土壤中镉污染的危害及其防治对策[J]. 云南农业大学学报, 2005, 20(3): 360-365.
[7]	朱琳, 闫宝龙, 韩国栋, 等. 不同载畜率对荒漠草原三种土壤酶活性的影响[J]. 中国草地学报, 2019, 41(3): 67-73.
[8]	张咏梅, 周国逸, 吴宁, 等. 土壤酶学的研究进展[J]. 热带亚热带植物学报, 2004, 12(1): 83-90.
[9]	Gu C, Zhang S, Han P, et al. Soil enzyme activity in soils subjected to flooding and the effect on nitrogen and phosphorus uptake by oilseed rape[J]. Frontiers in Plant Science, 2019, 10: 1-9. doi: 10.3389/fpls.2019.00001
[10]	Yu P, Tang X, Zhang A, et al. Responses of soil specific enzyme activities to short-term land use conversions in a salt-affected region, Northeastern China[J]. Science of the Total Environment, 2019, 687: 939-945. doi: 10.1016/j.scitotenv.2019.06.171
[11]	李玉瑛, 李冰. 柴油污染土壤生物修复对土壤酶活性的影响[J]. 生态环境学报, 2009, 18(5): 1753-1756.
[12]	García-Ruiz R, Ochoa V, Viñegla B, et al. Soil enzymes, nematode community and selected physico-chemical properties as soil quality indicators in organic and conventional olive oil farming: influence of seasonality and site features[J]. Applied Soil Ecology, 2009, 41(3): 305-314. doi: 10.1016/j.apsoil.2008.12.004
[13]	尹君, 高如泰, 刘文菊, 等. 土壤酶活性与土壤 Cd 污染评价指标[J]. 农业环境科学学报, 1999, 18(3): 130-132.
[14]	张甘霖, 龚子同. 土壤调查实验室分析方法 [M]. 北京: 科学出版社, 2012: 8-80.
[15]	梁小翠, 朱凡, 闫文德, 等. 马褂木盆栽土壤酶活性对 PAHs 胁迫的响应[J]. 中南林业科技大学学报, 2011, 31(5): 92-95, 116.
[16]	沈秋悦, 曹志强, 朱月芳, 等. 重金属 Cd 污染对土壤微生物活性影响的研究[J]. 环境污染与防治, 2016, 38(7): 11-14, 24.
[17]	杨惠芳, 王保军, 林力, 等. 单甲脒农药对土壤微生物种群和土壤酶活性的影响[J]. 应用与环境生物学报, 1996, 2(1): 58-65.
[18]	关松荫. 土壤酶及其研究法 [M]. 北京: 中国农业出版社, 1986: 294-332.
[19]	李阜棣, 喻子牛, 何绍江, 等. 农业微生物学实验技术 [M]. 北京: 中国农业出版社, 1996: 305-308.
[20]	Cordero I, Snell H, Bardgett R D, et al. High throughput method for measuring urease activity in soil[J]. Soil Biology and Biochemistry, 2019, 134: 72-77. doi: 10.1016/j.soilbio.2019.03.014 pmid: 31274933
[21]	Sinsabaugh R L, Shah J J F. Ecoenzymatic stoichiometry and ecological theory[J]. Annual Review of Ecology, Evolution, and Systematics, 2012, 43(1): 313-343. doi: 10.1146/ecolsys.2012.43.issue-1
[22]	姜必亮, 王伯荪, 蓝崇钰, 等. 垃圾填埋场渗滤液灌溉对土壤微生物生物量及酶活性的影响[J]. 环境科学学报, 2001, 21(1): 55-59.
[23]	Dindar E, Sagban F O T, Baskaya H S, et al. Variations of soil enzyme activities in petroleum-hydrocarbon contaminated soil[J]. International Biodeterioration & Biodegradation, 2015, 105: 268-275.
[24]	沈国清, 陆贻通, 洪静波, 等. 重金属和多环芳烃复合污染对土壤酶活性的影响及定量表征[J]. 应用与环境生物学报, 2005, 11(4): 479-482.
[25]	王佰华, 王军华, 孙儒瑞, 等. 超高效液相-荧光检测器法测定柴油中多环芳烃[J]. 应用化工, 2016, 45(9): 1789-1791.
[26]	贾蓉, 易维洁, 曲东, 等. TTC 法测定水稻土泥浆中脱氢酶活性的影响因素[J]. 西北农业学报, 2012, 21(4): 154-158.
[27]	赵连梅, 池勇志, 张春青, 等. TTC-脱氢酶活性测定中标准曲线的影响因素研究[J]. 实验室科学, 2009(4): 72-74.
[28]	Włodarczyk T, Stepniewski W, Brzezinska M. Dehydrogenase activity, redox potential, and emissions of carbon dioxide and nitrous oxide from cambisols under flooding conditions[J]. Biology and Fertility of Soils, 2002, 36: 200-206. doi: 10.1007/s00374-002-0513-1
[29]	Mersi M V, Sehinner F. An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride[J]. Biology and Fertility of Soils, 1991, 11(3): 216-220. doi: 10.1007/BF00335770
[30]	和文祥, 黄英锋, 朱铭莪, 等. 汞和镉对土壤脲酶活性影响[J]. 土壤学报, 2002, 39(3): 412-420.
[31]	Chaperon S, Sauve S. Toxicity interactions of cadmium, copper, and lead on soil urease and dehydrogenase activity in relation to chemical speciation[J]. Ecotoxicology and Environmental Safety, 2008, 70(1): 1-9. pmid: 18068781
[32]	张平, 甘国娟, 廖柏寒, 等. 铅锌矿区重金属复合污染对土壤酶活性的影响[J]. 安全与环境学报, 2013, 13(3): 147-150.
[33]	Hu X F, Jiang Y, Shu Y, et al. Effects of mining wastewater discharges on heavy metal pollution and soil enzyme activity of the paddy fields[J]. Journal of Geochemical Exploration, 2014, 147: 139-150. doi: 10.1016/j.gexplo.2014.08.001
[34]	吴家燕, 夏增禄, 巴音, 等. 土壤重金属污染的酶学诊断: 紫色土 Cd、 Cu、Pb 和 As 对水稻根系脱氢酶的影响[J]. 应用生态学报, 1991, 2(1): 58-62.
[35]	崔红标, 梁家妮, 范玉超, 等. 磷灰石等改良剂对铜污染土壤的修复效果研究: 对铜形态分布、土壤酶活性和微生物数量的影响[J]. 土壤, 2011, 43(2): 247-252.
[36]	Spedding T A, Hamel C, Mehuys G R, et al. Soil microbial dynamics in maize-growing soil under different tillage and residue management systems[J]. Soil Biology & Biochemistry, 2004, 36(3): 499-512. doi: 10.1016/j.soilbio.2003.10.026
[37]	Tao K Y, Zhang X Y, Chen X P, et al. Response of soil bacterial community to bioaugmentation with a plant residue-immobilized bacterial consortium for crude oil removal[J]. Chemosphere, 2019, 222: 831-838. doi: 10.1016/j.chemosphere.2019.01.133
[38]	李慧, 陈冠雄, 杨涛, 等. 沈抚灌区含油污水灌溉对稻田土壤微生物种群及土壤酶活性的影响[J]. 应用生态学报, 2005, 16(7): 1355-1359.
[39]	张晶, 张惠文, 张勤, 等. 长期石油污水灌溉对东北旱田土壤微生物生物量及土壤酶活性的影响[J]. 中国生态农业学报, 2008, 16(1): 67-70.
[40]	江伟, 于小飞, 田永兰, 等. 镉污染对文冠果土壤微生物的影响[J]. 江苏农业科学, 2018, 46(6): 228-231.
[41]	Baran S, Jolanta E, Oleszczuk P, et al. Enzymatic activity in an airfield soil polluted with polycyclic aromatic hydrocarbons[J]. Geoderma, 2004, 118(3/4): 221-232. doi: 10.1016/S0016-7061(03)00205-2

Effects of diesel fuel and Cd pollution on enzyme activity and microbial biomass in the soil

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 41

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHEN Jiayi, GAO Mingjing, LIU Yu, HUANG Xun, GUO Hao, ZHANG Xinying. Remediation of Cd-Zn-Cu contaminated river sediment by typical herbaceous plants [J]. Journal of Shanghai University（Natural Science Edition）, 2022, 28(1): 40-48.
[2]	QIN Aifang, XU Weifang, JIANG Lianghua. Analysis of radial consolidation of unsaturated soil with vertical drains considering drain resistance and the smear effect [J]. Journal of Shanghai University（Natural Science Edition）, 2021, 27(6): 1074-1084.
[3]	JIANG Yun, LU Ye, WANG Xiaoyong. Application of DIC technology in soil displacement analyses around model piles [J]. Journal of Shanghai University（Natural Science Edition）, 2021, 27(6): 1118-1127.
[4]	ZHANG Ying, MIN Jiahua, LIANG Xiaoyan, LIU Zhaoxin, LI Ming, ZHANG Jijun, ZHANG Jiaxuan, ZHANG Delong, SHEN Yue, WANG Linjun. Passivation composition analysis and passivation process optimization of CdZnTe [J]. Journal of Shanghai University（Natural Science Edition）, 2020, 26(4): 538-543.
[5]	Dean SUN, Zhou ZHANG, You GAO, Bo CHEN. Determination of basic parameters of SWCC for clayer soils [J]. Journal of Shanghai University（Natural Science Edition）, 2019, 25(6): 957-964.
[6]	LI Menglu, HU Xuefeng, DAI Huihui, LUO Fan, ZHANG Peifeng, AN Bonian. Effects of Brassica chinensis intercropping leguminous green manure on vegetable quality and soil enzyme activities [J]. Journal of Shanghai University（Natural Science Edition）, 2019, 25(2): 275-281.
[7]	CAO Yaozhong, YAO Wenjuan, CHENG Zekun. Analysis of soil pressure and displacement on bucket-based structure under reclamation silt [J]. Journal of Shanghai University（Natural Science Edition）, 2019, 25(2): 328-336.
[8]	ZHANG Zhao, SUN Dean, GAO You. Constitutive model for unsaturated sedimentary soils based on SFG model [J]. Journal of Shanghai University（Natural Science Edition）, 2019, 25(2): 337-346.
[9]	SHI Zhengchi, HE Chiquan, XIONG Pengpeng, CHENG Xue. Effect of SMS on the phytoremediation of Ricinus communis to Cd/Zn polluted soil [J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(6): 931-937.
[10]	LU Ye, SUN Hanqing, LI Hang. Application of modified DIC technology in press-in pile model test [J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(6): 993-1001.
[11]	LIU Feiyu, ZHANG Duanyang, WANG Jun. Cyclic and post-cyclic direct shear behavior of sandwich reinforcement-soil interface under different sand particle sizes [J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(3): 456-466.
[12]	WANG Sicong, SUN Dean. Time effect of shear strengths of unsaturated fly-ash and its prediction [J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(1): 108-117.
[13]	ZHANG Jun, LIN Yongliang. Micro-mechanism analysis of interface behavior of reinforced sand cushion with denti-inclusions [J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(1): 118-125.
[14]	QIN Aifang, Lü Kangli. Analysis of one-dimensional consolidation characteristics of viscoelastic unsaturated soils under cyclic loads [J]. Journal of Shanghai University（Natural Science Edition）, 2017, 23(6): 937-.
[15]	PENG Aixin, ZHANG Mengxi, ZHU Huachao. Triaxial test of high strength geocell reinforced soil [J]. Journal of Shanghai University（Natural Science Edition）, 2017, 23(4): 590-599.