荷叶生物碱对胰脂肪酶的抑制作用

上海大学学报(自然科学版)

荷叶生物碱对胰脂肪酶的抑制作用

朱晓青;吕敬慈;霍世欣

上海大学理学院，上海 200444

收稿日期:2006-03-20 出版日期:2007-02-28 发布日期:2007-02-28

Inhibitory Effect on Lipase of Alkaloid Derived from Lotus Leaf

ZHU Xiao qing;LU Jing ci;HUO Shi xin;et al

School of Sciences, Shanghai University, Shanghai 200444, China

Received:2006-03-20 Online:2007-02-28 Published:2007-02-28

摘要/Abstract

摘要： 荷叶粗粉经过石油醚去脂后，用95%乙醇提取，初步纯化后得荷叶生物碱粗提物.初步探讨了荷叶生物碱对胰脂肪酶的抑制机理：抑制剂不直接与酶分子作用，而是间接改变底物乳化来阻碍底物酶结合达到抑制作用.通过试验测定了抑制常数： K i=0.2 mg /m L.Dixon图和Lineweaver Burk图的特征表明，该抑制剂对胰脂肪酶的抑制呈非竞争性抑制.

关键词: 荷叶, 生物碱, 脂肪酶

Abstract: Ethanol extract of lotus leaves was first obtained, and the extract wa s then purified using chloroform. The effects of several factors on inhibitory a ctivity are studied. An inhibitory mechanism of the lotus leaf derived lipase i nhibitor is proposed. This inhibitor does not act directly on the lipase molecul e but blocks the enzyme substrate contact by modifying the emulsion of the lipi d substrate. The inhibitory constant ( K i) is 0.2 mg/mL. Based on Dixon plo ts and Linweaver Burk plots, the lipase inhibitor demonstrates uncompetitive inhibiti on for lipase.

Key words: alkaloid, lipase, lotus leaf

朱晓青;吕敬慈;霍世欣. 荷叶生物碱对胰脂肪酶的抑制作用[J]. 上海大学学报(自然科学版).

ZHU Xiao qing;LU Jing ci;HUO Shi xin;et al . Inhibitory Effect on Lipase of Alkaloid Derived from Lotus Leaf[J]. Journal of Shanghai University（Natural Science Edition）.