循环血miR-21-3p作为脓毒症心肌病的标记物

doi:10.3969/j.issn.1007-2861.2016.03.019

上海大学学报(自然科学版) ›› 2016, Vol. 22 ›› Issue (3): 366-370.doi: 10.3969/j.issn.1007-2861.2016.03.019

循环血miR-21-3p作为脓毒症心肌病的标记物

刘慧, 王惠, 史倞, 孔祥清

南京医科大学第一附属医院心脏科, 南京210029

收稿日期:2016-04-22 出版日期:2016-06-30 发布日期:2016-06-30
通讯作者: 孔祥清(1966—), 男, 教授, 主任医师, 博士生导师, 博士, 研究方向为先天性心脏病介入治疗. E-mail: xiangqingkong nj@163.com
作者简介:孔祥清(1966—), 男, 教授, 主任医师, 博士生导师, 博士, 研究方向为先天性心脏病介入治疗. E-mail: xiangqingkong nj@163.com
基金资助:
国家自然科学基金资助项目(81570247)

Circulating miR-21-3p as biomarkers for sepsis-induced cardiomyopathy

LIU Hui, WANG Hui, SHI Jing, KONG Xiangqing

Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China

Received:2016-04-22 Online:2016-06-30 Published:2016-06-30

摘要/Abstract

摘要：

已有研究发现微小RNA(microRNAs, miRNAs) 可由细胞分泌并释放进入循环系统, 在血清、血浆、尿液和其他体液中稳定存在, 并可作为非损伤性生物标记物对多种疾病进行早期诊断、鉴别分期、预后判断和疗效监测等. 与健康对照者相比, miR-21-3p 在22 例脓毒症心衰患者血浆中显著升高. 通过绘制受试者工作特征(receiver operating characteristic, ROC)曲线, 并分析其与临床参数的相关性发现, miR-21-3p 在鉴别脓毒症心衰和健康人群时, 曲线下面积(area under the curve, AUC)为0.949. 相关性分析结果显示, 血浆miR-21-3p水平与B 型脑钠肽(brain natriuretic peptide, BNP)(r = 0.968, P < 0.001)、心肌肌钙蛋白T (cardiac troponin T, cTNT)(r = 0.257, P = 0.002)水平呈正相关. 因此, 外周血中的miR-21-3p有作为脓毒症心肌病的生物标记物的潜力.

关键词: miR-21-3p , 循环miRNA, 脓毒症心肌病

Abstract:

Recently, microRNAs(miRNAs) are found to be abundantly present in body fluids including plasma and serum, and called circulating miRNAs. Increasing evidences suggest that circulating miRNAs may be useful as stable blood-based biomarkers in sepsis. Serum miR-21-3p is significantly increased in septic patients compared with healthy controls. Receiver operating characteristic (ROC) curve analyses suggest that these plasma miR-21-3p may be useful biomarkers for discriminating patients with sepsis-induced cardiomyopathy (SIC) from healthy controls. miR-21-3p yields the ROC area under the curve
(AUC) of 0.949. Further analyses indicate that the expression level of miR-21-3p is correlated with the brain natriuretic peptide (BNP) (r = 0.968, P < 0.001) and cardiac troponin T (cTNT) level (r = 0.257, P = 0.002). Serum miR-21-3p may serve as new biomarkers for sepsis with high specificity and sensitivity.

Key words: circulating miRNAs, miR-21-3p , sepsis-induced cardiomyopathy (SIC)

刘慧, 王惠, 史倞, 孔祥清. 循环血miR-21-3p作为脓毒症心肌病的标记物[J]. 上海大学学报(自然科学版), 2016, 22(3): 366-370.

LIU Hui, WANG Hui, SHI Jing, KONG Xiangqing. Circulating miR-21-3p as biomarkers for sepsis-induced cardiomyopathy[J]. Journal of Shanghai University（Natural Science Edition）, 2016, 22(3): 366-370.

参考文献

[1] Bone R C, Balk R A, Cerra F B, et al. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis [J]. Chest, 1992, 101(6): 1644-1655.
[2] Bone R C. Gram-negative sepsis. Background, clinical features, and intervention [J]. Chest, 1991, 100(3): 802-808.
[3] Martin G S, Mannino D M, Eaton S, et al. The epidemiology of sepsis in the United States from 1979 through 2000 [J]. New England Journal of Medicine, 2003, 348(16): 1546-1554.
[4] Merx M W, Weber C. Sepsis and the heart [J]. Circulation, 2007, 116(7): 793-802.
[5] Zaky A, Deem S, Bendjelid K, et al. Characterization of cardiac dysfunction in sepsis: an ongoing challenge [J]. Shock, 2014, 41(1): 12-24.
[6] Angus D C, Linde-Zwirble W T, Lidicker J, et al. Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care [J]. Critical Care
Medicine, 2001, 29(7): 1303-1310.
[7] Annane D, Bellissant E, Cavaillon J M. Septic shock [J]. Lancet, 2005, 365(9453): 63-78.
[8] Wang J F, Yu M L, Yu G, et al. Serum miR-146a and miR-223 as potential new biomarkers for sepsis [J]. Biochemical and Biophysical Research Communications, 2010, 394(1): 184-188.
[9] Wang L, Wang H C, Chen C, et al. Differential expression of plasma miR-146a in sepsis patients compared with non-sepsis-SIRS patients [J]. Experimental and Therapeutic Medicine, 2013, 5(4): 1101-1104.
[10] Roderburg C, Luedde M, Vargas C D, et al. Circulating microRNA-150 serum levels predict survival in patients with critical illness and sepsis [J]. PLoS One, 2013, 8(1): e54612.
[11] Wu S C, Yang J C, Rau C S, et al. Profiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture [J]. PLoS One, 2013, 8(1): e77936.
[12] Wang H, Zhang P, Chen W, et al. Evidence for serum miR-15a and miR-16 levels as biomarkers that distinguish sepsis from systemic inflammatory response syndrome in human subjects [J]. Clinical Chemistry and Laboratory Medicine, 2012, 50(8): 1423-1428.
[13] Tacke F, Roderburg C, Benz F, et al. Levels of circulating miR-133a are elevated in sepsis and predict mortality in critically ill patients [J]. Critical Care Medicine, 2014, 42(5): 1096-1104.
[14] Wang H, Meng K, Chen W, et al. Serum miR-574-5p: a prognostic predictor of sepsis patients [J]. Shock, 2012, 37(3): 263-267.
[15] Wang H, Bei Y, Shen S, et al. miR-21-3p controls sepsis-associated cardiac dysfunction via regulating SORBS2 [J]. Journal of Molecular and Cellular Cardiology, 2016, 94: 43-53.

循环血miR-21-3p作为脓毒症心肌病的标记物

Circulating miR-21-3p as biomarkers for sepsis-induced cardiomyopathy

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价