分散液液微萃取-高效液相色谱法联用检测豆浆中双酚A的质量浓度

doi:10.3969/j.issn.1007-2861.2015.03.010

上海大学学报(自然科学版) ›› 2016, Vol. 22 ›› Issue (6): 746-752.doi: 10.3969/j.issn.1007-2861.2015.03.010

分散液液微萃取-高效液相色谱法联用检测豆浆中双酚A的质量浓度

查一鸣, 王新秀, 范永, 王田霖, 罗立强

上海大学理学院, 上海 200444

收稿日期:2015-06-12 出版日期:2016-12-30 发布日期:2016-12-30
通讯作者: 罗立强(1973—), 男, 教授, 博士生导师, 博士, 研究方向为生物传感器、纳米电化学与电分析化学、直接甲醇燃料电池等. E-mail: luck@shu.edu.cn

Determination of bisphenol A in soybean milk by dispersive liquid-liquid microextraction coupled with high performance liquid chromatography

ZHA Yiming, WANG Xinxiu, FAN Yong, WANG Tianlin, LUO Liqiang

College of Sciences, Shanghai University, Shanghai 200444, China

Received:2015-06-12 Online:2016-12-30 Published:2016-12-30

摘要/Abstract

摘要：

建立一种采用分散液液微萃取-高效液相色谱法(dispersive liquid-liquid microextraction coupled with high performance liquid chromatography, DLLME-HPLC)联用对豆浆中的双酚A进行萃取和检测的新方法, 并考察不同因素对萃取回收率和富集倍数的影响. 实验结果表明, 在最优化的萃取条件下, 在1~100 μg/L质量浓度范围内, 双酚A的质量浓度与色谱峰面积呈良好的线性关系, 检出限为0.3 μg/L, 加标回收率在91.6%~97.5%之间, 相对标准偏差小于6%. 这说明该方法的准确度和重现性较好, 具有可行性, 适用于对市售豆浆饮品中双酚A质量含量的测定.

关键词: 豆浆, 高效液相色谱法 , 分散液液微萃取 , 双酚A

Abstract:

A novel method was developed for determination of bisphenol A in soybean milk using dispersive liquid-liquid microextraction coupled with high performance liquid chromatography (DLLME-HPLC). Under the optimal conditions, the peak area had a linear relation with the concentration of bisphenol A over the range of 1~100 μg/L. The detection limit was 0.3 μg/L and recovery was among 91.6%~97.5%. The method had good accuracy and reproducibility, and was appropriate for determination of bisphenol A in soybean milk.

Key words: dispersive liquid-liquid microextraction (DLLME) , high performance liquid chromatography (HPLC) , soybean milk, bisphenol A

查一鸣, 王新秀, 范永, 王田霖, 罗立强. 分散液液微萃取-高效液相色谱法联用检测豆浆中双酚A的质量浓度[J]. 上海大学学报(自然科学版), 2016, 22(6): 746-752.

ZHA Yiming, WANG Xinxiu, FAN Yong, WANG Tianlin, LUO Liqiang. Determination of bisphenol A in soybean milk by dispersive liquid-liquid microextraction coupled with high performance liquid chromatography[J]. Journal of Shanghai University（Natural Science Edition）, 2016, 22(6): 746-752.

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分散液液微萃取-高效液相色谱法联用检测豆浆中双酚A的质量浓度

Determination of bisphenol A in soybean milk by dispersive liquid-liquid microextraction coupled with high performance liquid chromatography

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