大气颗粒物对A549 和HUVEC 的DNA 损伤机制

doi:10.3969/j.issn.1007-2861.2013.04.015

上海大学学报(自然科学版) ›› 2013, Vol. 19 ›› Issue (4): 411-415.doi: 10.3969/j.issn.1007-2861.2013.04.015

大气颗粒物对A549 和HUVEC 的DNA 损伤机制

尚羽, 张玲, 范兰兰, 蒋玉婷

(上海大学环境与化学工程学院, 环境污染与健康研究所, 上海200444)

出版日期:2013-08-27 发布日期:2013-08-27
通讯作者: 尚羽(1982—), 女, 博士, 研究方向为大气污染物的区域污染特征和健康影响. E-mail: yushang@shu.edu.cn
作者简介:尚羽(1982—), 女, 博士, 研究方向为大气污染物的区域污染特征和健康影响. E-mail: yushang@shu.edu.cn
基金资助:
国家自然科学基金资助项目(21107068); 环保部环保公益性行业科研专项基金资助项目(201009032)

Genotoxicity of Size-Fractionated Ambient Particulate Matter in A549 and HUVEC Cell Lines

SHANG Yu, ZHANG Ling, FAN Lan-lan, JIANG Yu-ting

(Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering,
Shanghai University, Shanghai 200444, China)

Online:2013-08-27 Published:2013-08-27

摘要/Abstract

摘要： 采集北京市海淀区大气颗粒物粗颗粒(PM_10−2.5)、细颗粒( PM_2.5−0.1 ) 和超细颗粒(PM_0.1),分析颗粒物对人肺上皮细胞(human lung epithelial cell, A549) 和人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)的基因毒性及促进活性氧自由基(reactive oxygen species, ROS) 生成的机制. 彗星实验发现: PM_10−2.5, PM_2.5−0.1 和PM_0.1 对2 种细胞均有显著的基因毒性, 并呈剂量-效应关系; 细和超细颗粒造成的DNA 损伤显著高于粗颗粒; HUVEC 细胞的DNA 损伤程度大于A549 细胞; PM_2.5−0.1 和PM_0.1 可诱导2 种细胞内ROS 水平显著升高, 而PM_10−2.5 不能. 因此细胞内DNA 损伤可能与ROS 生成有一定联系.

关键词: DNA 损伤, 大气颗粒物, 活性氧自由基, 人肺上皮细胞(A549), 人脐静脉血管内皮细胞(HUVEC)

Abstract: Ambient particulate matter has become one of the most harmful pollutants in the air. The genotoxic effects and generationmechanism of reactive oxygen species (ROS) were studied in A549 and HUVEC cells treated with three sizes of ambient particles, PM_10−2.5, PM_2.5−0.1and PM_0.1. The results from comet assay show that PM_10−2.5, PM_2.5−0.1 and PM_0.1 caused significant DNA damages in both A549 and HUVEC cells in a dose-dependent manner. In both types of cells, the DNA damage caused by PM_2.5−0.1 and PM_0.1are significantly higher than that caused by PM_10−2.5, and PMs cause higher DNA damage in HUVEC cells than that in A549 cells. PM_2.5−0.1 and PM_0.1 can significantly increase the intracellular ROS level, but PM_10−2.5cannot. DNA damage in cells induced by particles may be related to the generation of ROS, which deserves further investigations.

Key words: ambient particulate matter, DNA damage, human lung epithelial cell (A549), human umbilical vein endothelial cell (HUVEC), ROS generation

中图分类号:

R 994.6

尚羽, 张玲, 范兰兰, 蒋玉婷. 大气颗粒物对A549 和HUVEC 的DNA 损伤机制[J]. 上海大学学报(自然科学版), 2013, 19(4): 411-415.

SHANG Yu, ZHANG Ling, FAN Lan-lan, JIANG Yu-ting. Genotoxicity of Size-Fractionated Ambient Particulate Matter in A549 and HUVEC Cell Lines[J]. Journal of Shanghai University（Natural Science Edition）, 2013, 19(4): 411-415.

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大气颗粒物对A549 和HUVEC 的DNA 损伤机制

Genotoxicity of Size-Fractionated Ambient Particulate Matter in A549 and HUVEC Cell Lines

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