上海春季大气颗粒物中致敏悬铃木花粉蛋白的分布特征

doi:10.12066/j.issn.1007-2861.2007

上海大学学报(自然科学版) ›› 2019, Vol. 25 ›› Issue (4): 544-549.doi: 10.12066/j.issn.1007-2861.2007

上海春季大气颗粒物中致敏悬铃木花粉蛋白的分布特征

赵慧¹, 彭加仙¹, 洪强², 张红莉², 王伟倩³, 王青躍³, 谭正莹¹, 周树敏², 张卫², 吕森林¹()

1. 上海大学环境与化学工程学院, 上海 200444
2. 上海大学生命科学学院, 上海 200444
3. 日本埼玉大学大学院理工学研究科, 埼玉 338-8570

收稿日期:2017-12-21 出版日期:2019-08-30 发布日期:2019-09-04
通讯作者: 吕森林 E-mail:senlinlv@staff.shu.edu.cn
基金资助:
国家自然科学基金资助项目(21477073);国家自然科学基金资助项目(41511140096)

Distributions of allergenic platanus pollen protein in the ambient particulates in the spring of Shanghai

Hui ZHAO¹, Jiaxian PENG¹, Qiang HONG², Hongli ZHANG², Weiqian WANG³, Qingyao WANG³, Zhengying TAN¹, Shumin ZHOU², Wei ZHANG², Senlin LÜ¹()

1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
2. School of Life Sciences, Shanghai University, Shanghai 200444, China
3. School of Science and Engineering, Saitama University, Saitama 338-8570, Japan

Received:2017-12-21 Online:2019-08-30 Published:2019-09-04
Contact: Senlin LÜ E-mail:senlinlv@staff.shu.edu.cn

摘要/Abstract

摘要：

选择上海大气颗粒物中悬铃木致敏花粉蛋白Pla a3为研究对象, 利用生物工程手段表达Pla a3蛋白, 并利用该致敏蛋白免疫大鼠, 制备出单一致敏原的抗体. 通过酶联免疫吸附测定法(enzyme linked immunosorbent assay, ELISA), 检测分析上海大气颗粒物中Pla a3的分布特征. 结果表明: 颗粒物中总蛋白质量浓度在$<$1.1 $\mu $m的粒径段内最高, 为2.5$\sim $3.0 $\mu $g/m$^{3}$; Pla a3蛋白主要分布在$>$7.0 $\mu $m的粒径段内, 其平均质量浓度为7.5 pg/m$^{3}$, 但在$<$1.1 $\mu $m粒径段内的质量浓度最低. 在上述分析结果基础上, 将进一步探讨颗粒物中主要有害组分与致敏蛋白分布之间的关系.

关键词: 大气颗粒物, 致敏蛋白Pla a3, 酶联免疫吸附测定法, 粒径分布

Abstract:

The allergenic pollen protein Pla a3 in Shanghai atmospheric particulate matter was selected as the research aim. Pla a3 protein from platanus pollen was expressed by biological engineering. Then rats were immunized and special antibody were prepared from the rat serum. The distribution of Pla a3 protein in Shanghai atmospheric particulate matter was maeasured and analyzed by making use of the enzyme linked immunosorbent assay (ELISA). Research results showed that total protein content in particulate matter was the highest in the particles with size diameter less than 1.1 $\mu$m. Its mass concentration is 2.5$\sim$3.0 $\mu$g/m$^3$. The Pla a3 protein in particulate matters was found mainly in particles with size diameter larger than 7.0 $\mu$m, with average mass level was 7.5 pg/m$^3$. Mass level of Pla a3 protein with size diameter less than 1.1 $\mu$m was the least. Based on the research data collected, the connection between allergenic protein and toxic composition in the ambient particles will be further explored.

Key words: atmospheric particulate matter, allergenic protein Pla a3, enzyme linked immunosorbent assay (ELISA), size distribution

中图分类号:

X820

赵慧, 彭加仙, 洪强, 张红莉, 王伟倩, 王青躍, 谭正莹, 周树敏, 张卫, 吕森林. 上海春季大气颗粒物中致敏悬铃木花粉蛋白的分布特征[J]. 上海大学学报(自然科学版), 2019, 25(4): 544-549.

Hui ZHAO, Jiaxian PENG, Qiang HONG, Hongli ZHANG, Weiqian WANG, Qingyao WANG, Zhengying TAN, Shumin ZHOU, Wei ZHANG, Senlin LÜ. Distributions of allergenic platanus pollen protein in the ambient particulates in the spring of Shanghai[J]. Journal of Shanghai University（Natural Science Edition）, 2019, 25(4): 544-549.

图/表 3

参考文献 18

[1]	D'amato G, Liccardi G . The increasing trend of seasonal respiratory allergy in urban areas[J]. Allergy, 2002,57(71):35.
[2]	Hashiguchi K, Tang T H, Tsubaki S , et al. Preliminary study on Japanese cedar pollinosis in an artificial exposure chamber (Ohio Chamber)[J]. Allergology International, 2007,56(2):125-130.
[3]	Bacsi A, Choudhury B N, Sur S , et al. Subpollen particles: carriers of allergenic proteins and oxidases[J]. Journal of Allergy & Clinical Immunology, 2006,118(4):844-850.
[4]	程晟, 余咏梅, 阮标 . 中国主要城市气传花粉植物种类与分布[J]. 中华临床免疫和变态反应杂志, 2015(2):136-141.
[5]	Asturias J A, Ibarrola I, Eraso E, et al. The major Platanus acerifolia pollen allergen Pla a1 has sequence homology to invertase inhibitors[J]. Clinical & Experimental Allergy Journal of the British Society for Allergy & Clinical Immunology, 2003,33(7):978.
[6]	Ibarrola I, Arilla M C, Martinez A, et al. Identification of a polygalacturonase as a major allergen (Pla a2) from Platanus acerifolia pollen[J]. Journal of Allergy & Clinical Immunology, 2004,113(6):1185-91.
[7]	Lauer I, Miguelmoncin M S, Abel T , et al. Identification of a plane pollen lipid transfer protein (Pla a3) and its immunological relation to the peach lipid-transfer protein, Prup3[J]. Clinical & Experimental Allergy, 2007,37(2):261.
[8]	Chen Z, Yang Y, Chen X , et al. Characterization of two pollen allergens of the London plane tree in Shanghai[J]. Iranian Journal of Allergy Asthma & Immunology, 2015,14(2):139.
[9]	辛嘉楠, 欧阳志云, 郑华 , 等. 城市化加剧花粉过敏症的机制研究进展[J]. 环境与健康杂志, 2007,24(10):833-836.
[10]	王强翔, 谭正莹, 赵慧 , 等. 宣威肺癌高发区燃煤排放颗粒物中铁的价态及其氧化性[J]. 环境科学, 2017,38(6):2273-2279.
[11]	陈小东, 陈臻, 张尚权 , 等. 梧桐花粉致敏大鼠动物模型的建立及特异性抗体检测[J]. 中国细胞生物学学报, 2004,26(5):523-525.
[12]	邢锐, 齐名, 姚凯 , 等. 二球悬铃木花粉主要变应原蛋白编码基因克隆的制备[J]. 南京医科大学学报(自然科学版), 2006,26(12):1150-1154.
[13]	Franze T, Weller M G, Reinhard N A , et al. Protein nitration by polluted air[J]. Environmental Science & Technology, 2005,39(6):1673.
[14]	Lee B K, Jun N Y, Lee H K . Comparison of particulate matter characteristics before, during, and after Asian dust events in Incheon and Ulsan, Korea[J]. Atmospheric Environment, 2004,38(11):1535-1545.
[15]	Lü S L, Yi F, Hao X J , et al. Physicochemical properties and ability to generate free radicals of ambient coarse, fine, and ultrafine particles in the atmosphere of Xuanwei, China, an area of high lung cancer incidence[J]. Atmospheric Environment, 2014,97:519-528.
[16]	吕森林, 王青躍, 吴明红 , 等. 城区飞散致敏花粉与大气细/超细颗粒物的协同生物效应研究[J]. 环境科学, 2010,31(9):2260-2266.
[17]	Kang H, Xie Z, Hu Q . Ambient protein concentration in PM10 in Hefei, central China[J]. Atmospheric Environment, 2012,54(5):73-79.
[18]	Fern$\acute{A}$ndez-Gonz$\acute{A}$lez M, Guedes A, Abreu I , et al. Pla a_1 aeroallergen immunodetection related to the airborne platanus pollen content[J]. Science Total Environment, 2013,463:855-860.

上海春季大气颗粒物中致敏悬铃木花粉蛋白的分布特征

Distributions of allergenic platanus pollen protein in the ambient particulates in the spring of Shanghai

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