蛋白质-蛋白质相互作用的实验检测方法

doi:10.12066/j.issn.1007-2861.2065

摘要/Abstract

摘要：

生物体内的蛋白质分子常常通过与其他蛋白质分子发生相互作用来发挥其生物功能. 因此, 研究蛋白质-蛋白质相互作用 (protein-protein interaction, PPI) 对于阐明蛋白质分子的生物功能以及分子作用机理具有重要的意义. 主要介绍基于生物物理和生物化学原理的检测蛋白质-蛋白质相互作用的实验研究方法, 并对发展趋势做出展望.

关键词: 蛋白质-蛋白质相互作用, 生物物理方法, 生物化学方法

Abstract:

Protein molecules usually interact with other partner protein molecules to execute their biological functions. Hence, the characterisation of protein-protein interac- tions (PPIs) plays a significant role inelucidating the molecular mechanism of proteins. With the progress of proteomics comes the development of a variety of experimental methods for characterising protein-protein interactions. This article mainly reviews recently developed biophysical- or biochemical-basedexperimental methods for characterising protein-protein interactions.

Key words: protein-protein interactions (PPIs), biochemical methods, biophysical methods

中图分类号:

Q533

孔文娜, 周宓, 林海霞, 王任小. 蛋白质-蛋白质相互作用的实验检测方法[J]. 上海大学学报(自然科学版), 2021, 27(1): 106-116.

KONG Wenna, ZHOU Mi, LIN Haixia, WANG Renxiao. Experimental methods for characterizing protein-protein interactions[J]. Journal of Shanghai University（Natural Science Edition）, 2021, 27(1): 106-116.

图/表 3

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