乌江流域某水库浮游藻类群落结构及多样性分析

doi:10.12066/j.issn.1007-2861.2085

上海大学学报(自然科学版) ›› 2021, Vol. 27 ›› Issue (1): 97-105.doi: 10.12066/j.issn.1007-2861.2085

乌江流域某水库浮游藻类群落结构及多样性分析

岳一鸿¹, 傅志伟¹, 陈学萍¹, 杨明¹(), 王宝利², 汪福顺¹

1.上海大学环境与化学工程学院, 上海 200444
2.天津大学表层地球系统科学研究院, 天津 300072

收稿日期:2018-07-09 出版日期:2021-02-28 发布日期:2020-01-10
通讯作者: 杨明 E-mail:mingyang@shu.edu.cn
作者简介:杨明(1979---), 女, 研究员, 博士, 研究方向为环境生态学. E-mail: mingyang@shu.edu.cn.
基金资助:
国家重点研发计划资助项目(2016YFA0601001)

Community structure and diversity phytoplankton in the Wujiang River Basin reservoir

YUE Yihong¹, FU Zhiwei¹, CHEN Xueping¹, YANG Ming¹(), WANG Baoli², WANG Fushun¹

1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
2. Institute of Surface-Earth System Science, Tianjin University, Tianjin 300072, China

Received:2018-07-09 Online:2021-02-28 Published:2020-01-10
Contact: YANG Ming E-mail:mingyang@shu.edu.cn

摘要/Abstract

摘要：

浮游藻类在河流生态系统中发挥着重要的生态功能. 为探明乌江流域不同梯级水库中浮游藻类的种群结构特征, 采集了乌江流域某水库水深分别为 0, 10 和 45 m 的水样过滤膜分层样本, 利用两对浮游藻类通用简并引物对水样进行 23S rRNA 基因扩增、扩增片段建库和高通量测序分析. 通过对两对引物获得的浮游藻类种群覆盖度比较, 选择出了相对高效的分子标记, 获得了不同水深水体中浮游藻类群落结构组成的初步信息, 为进一步深入研究乌江流域不同梯级水库中浮游藻类物种多样性提供了一种新手段.

关键词: 乌江流域, 浮游藻类, 简并引物, 高通量测序

Abstract:

Phytoplankton plays a crucial role in river ecosystems. To characterise the structure of phytoplankton communities in cascade reservoirs of the Wujiang River Basin, filter samples were collected at 0, 10, and 45 m depth, and two previously reported degenerate primers were applied to amplify the 23S rRNA gene fragment and construct the library for high-throughput sequencing. An efficient identification method was developed through comparison of species coverage between two degenerate primers, and primary information was obtained on the phytoplankton community diversity. This study provides a powerful tool for potential studies on the ecological effect of damming on phytoplankton communities in riverine ecosystems.

Key words: Wujiang River Basin, phytoplankton, degenerate primer, high-throughput sequencing

中图分类号:

Q178.51

岳一鸿, 傅志伟, 陈学萍, 杨明, 王宝利, 汪福顺. 乌江流域某水库浮游藻类群落结构及多样性分析[J]. 上海大学学报(自然科学版), 2021, 27(1): 97-105.

YUE Yihong, FU Zhiwei, CHEN Xueping, YANG Ming, WANG Baoli, WANG Fushun. Community structure and diversity phytoplankton in the Wujiang River Basin reservoir[J]. Journal of Shanghai University（Natural Science Edition）, 2021, 27(1): 97-105.

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乌江流域某水库浮游藻类群落结构及多样性分析

Community structure and diversity phytoplankton in the Wujiang River Basin reservoir

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