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Journal of Shanghai University >

2019 , Vol. 25 >Issue 1: 164 - 170

DOI: https://doi.org/10.12066/j.issn.1007-2861.1894

Research Articles

Directional migration of neural stem cells induced by orderly gradient scaffolds

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  • 1. School of Life Sciences, Shanghai University, Shanghai 200444, China
    2. Key Laboratory for Nano-Bio Interfaces Research, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Science, Suzhou 215123, Jiangsu, China
    3. Key Laboratory of Hui Ethnic Medicine Modernization Ministry of Education, Ningxia Medical University, Yinchuan 750004, China

Received date: 2017-03-04

  Online published: 2019-02-26

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Abstract

Stromal-cell-derived factor-1$\alpha $ (SDF1$\alpha )$ plays an important role in inducing stem cell migration. Using a modified electrospinning technique, CBD-SDF1$\alpha $ embedded in radial orderly gradient fiber scaffolds to study migration of neural stem cells (NSCs) and neural stem cell spheres (NSCS) was prepared. The following results were obtained. 1) Gradient scaffolds induced migration of NSCs and NSCS along the orderly fiber scaffolds from a low concentration area to a high concentration area of collagen-binding domain SDF1$\alpha $ (CBD-SDF1$\alpha $). 2) The number and diffusion area in NSCs derived from NSCS were increased significantly in a high concentration area than in a low concentration area of CBD-SDF1$\alpha $. 3) NSCS exhibited polarized morphology and extended directionally along aligned gradient fiber scaffolds.

Key words： neural stem cells (NSCs); neural stem cell sphere (NSCS); cell migration

Cite this article

LI Mengyuan, LI Xiaoran, DAI Jianwu, WEN Tieqiao . Directional migration of neural stem cells induced by orderly gradient scaffolds[J]. Journal of Shanghai University, 2019 , 25(1) : 164 -170 . DOI: 10.12066/j.issn.1007-2861.1894

References

[1] Imitola J, Raddassi K, Park K I , et al. Directed migration of neural stem cells to sites of CNS injury by the stromal cell-derived factor 1$\alpha$/CXC chemokine receptor 4 pathway[J]. Proceedings of the National Academy of Sciences, 2004,101(52):18117-18122.
[2] Han J, Dai J W, Chen L , et al. Maintenance of the self-renewal properties of neural progenitor cells cultured in three-dimensional collagen scaffolds by the REDD1-mTOR signal pathway[J]. Biomaterials, 2013,34(8):1921-1928.
[3] Shi J J, Sun J, Zhang J , et al. Demineralized bone matrix scaffolds modified by CBD-SDF1$\alpha $ promote bone regeneration via recruiting endogenous stem cells[J]. ACS Applied Materials and Interfaces, 2016,8(41):27511-27522.
[4] Ellison G M, Nadal-Ginard B, Torella D , et al. Optimizing cardiac repair and regeneration through activation of the endogenous cardiac stem cell compartment[J]. Journal of Cardiovascular Translational Research, 2012,5(5):667-677.
[5] Fatemeh P, Zarch B, Yazdi B Y , et al. Application of stem cell/growth factor system, as a multimodal therapy approach in regenerative medicine to improve cell therapy yields[J]. International Journal of Cardiology, 2014,173(1):12-19.
[6] Li X R, Xie J W, Justin L , et al. Nanofiber scaffolds with gradations in mineral content for mimicking the tendon-to-bone insertion site[J]. Nano Letters, 2009,9(7):2763-2768.
[7] Filippo T R M, Galindo L T, Barnabe G F , et al. CXCL12 N-terminal end is sufficient to induce chemotaxis and proliferation of neural stem/progenitor cells[J]. Stem Cell Research, 2013,11(2):913-925.
[8] Liang H, Li X, Chen B , et al. A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy[J]. Journal of Controlled Release, 2015,209(10):101-109.
[9] Li X, Liang H, Sun J , et al. Electrospun collagen fibers with spatial patterning of SDF1$\alpha$ for the guidance of neural stem cells[J]. Advanced Healthcare Materials, 2015,4(12):1869-1876.
[10] Li X R, Li M Y, Sun J , et al. Radially aligned electrospun fibers with continuous gradient of SDF1$\alpha $ for the guidance of neural stem cells[J]. Small, 2016,12(36):5009-5018.
[11] Kim H D, Peyton S R . Bio-inspired materials for parsing matrix physicochemical control of cell migration: a review[J]. Integrative Biology, 2012,4(1):37-52.
[12] Li M, Hale J S, Rich J N , et al. Chemokine CXCL12 in neurodegenerative diseases: an SOS signal for stem cell-based repair[J]. Trends in Neurosciences, 2012,35(10):619-628.
[13] Andreas K, Sittinger M, Ringe J , et al. Toward in situ tissue engineering: chemokine-guided stem cell recruitment[J]. Trends in Biotechnology, 2014,32(9):483-492.
[14] Ratajczak M Z, Kim C H, Abdel-Latif A , et al. A novel perspective on stem cell homing and mobilization: review on bioactive lipids as potent chemoattractants and cationic peptides as underappreciated modulators of responsiveness to SDF-1 gradients[J]. Leukemia, 2012,26(1):63-72.
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