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Journal of Shanghai University >

2018 , Vol. 24 >Issue 3: 476 - 485

DOI: https://doi.org/10.12066/j.issn.1007-2861.1822

Research Paper

Effect of different Numb isoforms on proliferation and migration abilities of breast cancer cell line MCF-7

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  • 1. School of Life Sciences, Shanghai University, Shanghai 200444, China
    2. SARI Center for Stem Cell and Nanomedicine, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
    3. SARITEX Center for Stem Cell Engineering Translational Medicine, Eastern Hospital Affiliated to Tongji University, Shanghai 200085, China

Received date: 2016-06-21

  Online published: 2018-06-28

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Abstract

Objective This study explores the role of Numb isoforms in proliferation and migration of breast cancer cells. Methods TALEN technology was used to knock out Numb gene in MCF-7 cells, and then transfected p65, p66, p71 and p72 expression vectors into these Numb-KO MCF-7 cells. On this occasion, CCK-8 proliferation assay and wound scratch assay were used to detect MCF-7 cell proliferation and migration. Results The results show that transcription activator-like effector nuclease (TALEN) is effective in knocking out Numb gene. Compared with normal MCF-7 cells, proliferation and migration abilities of Numb-KO MCF-7 cells are significantly enhanced. Numb isoform p72 inhibits proliferation of Numb-KO MCF-7 cells, and Numb isoforms p65, p66, p71 and p72 inhibit migration of Numb-KO MCF-7 cells.

Key words： breast cancer; Numb gene; isoform; cell proliferation; cell migration

Cite this article

SU Hang, WANG Baolei, LI Huashun, ZHU Chenguang . Effect of different Numb isoforms on proliferation and migration abilities of breast cancer cell line MCF-7[J]. Journal of Shanghai University, 2018 , 24(3) : 476 -485 . DOI: 10.12066/j.issn.1007-2861.1822

References

[1] P ece S, C onfalonieri S, R r P, et al. Numb-ing down cancer by more than just a Notch[J]. Biochimica et Biophysica Acta, 2011,1815(1):26-43.
[2] W ang Z, Li S S C. Numb: a new player in EMT[J]. Cell Adhesion and Migration, 2010,4(2):176-179.
[3] D ing X, M a M, T eng J, et al. Numb induces E-cadherin adhesion dissolution, cytoskeleton reorganization, and migration in tubular epithelial cells contributing to renal fibrosis[J]. Current Molecular Medicine, 2015,15(4):368-379.
[4] T osoni D, Z ecchini S, C oazzoli M, et al. The Numb/p53 circuitry couples replicative self-renewal and tumor suppression in mammary epithelial cells[J]. Journal of Cell Biology, 2015,211(4):845-862.
[5] P ece S, S erresi M, S antolini E, et al. Loss of negative regulation by Numb over Notch is relevant to human breast carcinogenesis[J]. Journal of Cell Biology, 2004,167(2):215-221.
[6] D i M L, F erretti E, G reco A, et al. Numb is a suppressor of Hedgehog signalling and targets Gli1 for itch-dependent ubiquitination[J]. Nature Cell Biology, 2006,8(12):1415-1423.
[7] S hao X, L iu Y, Y u Q, et al. Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b[J]. Cell Research, 2016,26(5):593-612.
[8] S un M, A sghar S Z, Z hang H. The polarity protein Par3 regulates APP trafficking and processing through the endocytic adaptor protein Numb[J]. Neurobiology of disease, 2016,93(9):1-11.
[9] D ho S E, F rench M B, W oods S A, et al. Characterization of four mammalian Numb protein isoforms. Identification of cytoplasmic and membrane-associated variants of the phosphotyrosine binding domain[J]. Journal of Biological Chemistry, 1999,274(46):33097-33104.
[10] 钱文雨, 李华顺. Numb与神经发育[J]. 中国细胞生物学学报, 2010(1):1-16.
[11] B ani-Y aghoub M, K ubu C J, C owling R, et al. A switch in Numb isoforms is a critical step in cortical development[J]. Developmental Dynamics, 2007,236(3):696-705.
[12] V erdi J M, B ashirullah A, G oldhawk D E, et al. Distinct human Numb isoforms regulate differentiation vs. proliferation in the neuronal lineage[J]. Proceedings of the National Academy of Sciences of USA, 1999,96(18):10472-10476.
[13] S leeman J P, T hiery J P. SNAPSHOT: the epithelial-mesenchymal transition[J]. Cell, 2011,145(1):162-162.
[14] G ulino A, D i M L, S crepanti I. The multiple functions of Numb[J]. Experimental Cell Research, 2009,316(6):900-906.
[15] K araczyn A, B aniyaghoub M, T remblay R, et al. Two novel human Numb isoforms provide a potential link between development and cancer[J]. Neural Development, 2010,5(12):1040-1048.
[16] V erdi J M, B ashirullah A, G oldhawk D E. Distinct human Numb isoforms regulate differentiation vs. proliferation in the neuronal lineage[J]. Proceedings of the National Academy of Sciences, 1999,96(18):10472-10476.
[17] T oriya M, T okunaga A, S awamoto K, et al. Distinct functions of human Numb isoforms revealed by misexpression in the neural stem cell lineage in the Drosophila larval brain[J]. Developmental Neuroscience, 2005,28(1/2):142-155.
[18] G uo M, J an L Y, J an Y N. Control of daughter cell fates during asymmetric division: interaction of Numb and Notch[J]. Neuron, 1996,17(1):27-41.
[19] D ho S E, J acob S, W olting C D, et al. The mammalian Numb phosphotyrosine-binding domain. Characterization of binding specificity and identification of a novel PDZ domain-containing Numb binding protein, LNX[J]. Journal of Biological Chemistry, 1998,273(15):9179-9187.
[20] W illiamson M P. The structure and function of proline-rich regions in proteins[J]. Biochemical Journal, 1994,297(2):249-260.
[21] B erdnik D, T orok T G M, K noblich J A. The endocytic protein alpha-adaptin is required for Numb-mediated asymmetric cell division in Drosophila[J]. Developmental Cell, 2002,3(2):221-231.
[22] R asin M R, G azula V R, B reunig J J, et al. Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors[J]. Nature Neuroscience, 2007,10(7):819-827.
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