Ambient particulate matter has become one of the most harmful pollutants in the air. The genotoxic effects and generationmechanism of reactive oxygen species (ROS) were studied in A549 and HUVEC cells treated with three sizes of ambient particles, PM10−2.5, PM2.5−0.1 and PM0.1. The results from comet assay show that PM10−2.5, PM2.5−0.1 and PM0.1 caused significant DNA damages in both A549 and HUVEC cells in a dose-dependent manner. In both types of cells, the DNA damage caused by PM2.5−0.1 and PM0.1are significantly higher than that caused by PM10−2.5, and PMs cause higher DNA damage in HUVEC cells than that in A549 cells. PM2.5−0.1 and PM0.1 can significantly increase the intracellular ROS level, but PM10−2.5 cannot. DNA damage in cells induced by particles may be related to the generation of ROS, which deserves further investigations.
SHANG Yu, ZHANG Ling, FAN Lan-lan, JIANG Yu-ting
. Genotoxicity of Size-Fractionated Ambient Particulate Matter in A549 and HUVEC Cell Lines[J]. Journal of Shanghai University, 2013
, 19(4)
: 411
-415
.
DOI: 10.3969/j.issn.1007-2861.2013.04.015
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