A novel microstrip rectifying circuit is proposed in this paper. The rectifying circuit is analyzed and designed with the software ADS 2006A. Simulation results show that the rectifying circuit obtains 81.4% conversion efficiency from microwave to DC power on a load of 320 Ω at 5.80 GHz. The bandwidth of conversion efficiency more than 75% is 300 MHz. Experiment results indicate that the highest voltage of 4.57 V is measured on a load of 298 Ω with conversion efficiency of 68.5%. The rectifying circuit has advantages of compact structure, high conversion efficiency and ease of integration. It can be applied to energy supply for radio frequency identification (RFID) systems and wireless sensors.