To investigate the efficacy of plant-derived extracellular vesicles (PDEVs) loaded with angiotensin-converting enzyme 2 (ACE2) in mitigating respiratory system dam-age induced by particulate matter (PM_2.5), we extracted PDEVs from grapes and loaded them with ACE2 overexpression plasmids. The effect of the modified PDEVs on human bronchial epithelial cells (Beas-2B) after exposure to PM_2.5 was investigated using CCK8 assays, dihydroethidium (DHE) staining, western blotting, and real-time quantitative poly-merase chain reaction (PCR). Exposure to PM_2.5 increases oxidative stress, apoptosis, and inflammatory factor production in Beas-2B cells. PDEVs loaded with ACE2 effectively delivered ACE2 to cells and thereby alleviated PM_2.5-induced cell damage including apop-tosis, oxidative stress, and inflammation. PDEVs thus show potential for use as carriers of genes that can prevent apoptosis and damage caused by atmospheric PM_2.5 exposure.