中文

Journal of Shanghai University >

2020 , Vol. 26 >Issue 2: 227 - 233

DOI: https://doi.org/10.12066/j.issn.1007-2861.2078

Research Articles

Analysis of cembratriene-diols in tobacco by LC-GC/MS

Expand
  • 1. Technical Center, Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China
    2. School of Materials Science and Engineering, Shanghai University, Shanghai 200444, China

Received date: 2018-08-15

  Online published: 2020-04-29

Fold

Abstract

Cembratriene-diols are the precursors of important neutral aroma components and their derivatives, which affect significantly the aroma style and tobacco quality. In this study, a method of in-series combination of ultrasound-assisted extraction and online liquid chromatography-gas chromatography/mass spectrometry (LC-GC/MS) was constructed for the analysis of cembratriene-diols in tobacco. Cembratriene-diols were first extracted by ultrasound-assisted extraction and then on-line clean-up and analysis by online LC-GC/MS system. The pretreatment was highly efficient and easy-handling. Good recovery and reproducibility had also been achieved. This method was subsequently applied to the analysis of cembratriene-diols extracted from different regions of tobacco leaves. The satisfactory performance indicated that this strategy was suitable for the high-throughput analysis of cembratriene-diols in tobacco.

Key words： tobacco leaf; cembratriene-diols; silylation derivatization; liquid chromatography-gas chromatography/mass spectrometry (LC-GC/MS)

Cite this article

HUANG Zhi, BI Yanjiu, SHA Yunfei, XIE Wenyan, WU Da, LIU Baizhan, LIU Taiang . Analysis of cembratriene-diols in tobacco by LC-GC/MS[J]. Journal of Shanghai University, 2020 , 26(2) : 227 -233 . DOI: 10.12066/j.issn.1007-2861.2078

References

[1] 韩锦峰, 张志勇, 刘华山 , 等. 烟草腺毛及其分泌物西柏三烯醇类物质的研究进展[J]. 中国烟草学报, 2013,19(5):118-124.
[2] Severson R F, Arrendale R F, Chortyk O T , et al. Quantitation of the major cuticular components from green leaf of different tobacco types[J]. Journal of Agricultural Food Chemistry, 1984,32(3):566-570.
[3] Johnson A W, Severson R F, Hudson J , et al. Tobacco leaf trichomes and their exudates[J]. Tob Sci, 1985,29:67-72.
[4] 景延秋, 宫长荣, 张月华 , 等. 烟草香味物质分析研究进展[J]. 中国烟草科学, 2005(2):44-48.
[5] 韩锦峰, 王广山, 远彤 , 等. 烤烟叶面分泌物的初步研究[J]. 中国烟草科学, 1995(2):10-13.
[6] 任晋, 苏亚伦 . 西松烷型大环二萜类化合物研究进展[J]. 中草药, 2014,45(20):2997-3007.
[7] 李国强, 张艳玲, 林文翰 . 西松烷二萜类海洋活性成分研究进展[J]. 中国海洋大学学报, 2006,36(3):370-376.
[8] 黄建设, 龙丽娟, 张偲 . 海洋天然产物及其生理活性的研究进展[J]. 海洋通报, 2001,20(4):83-91.
[9] 王冬, 张小全, 杨铁钊 , 等. 类西柏烷二萜代谢机理及调控研究进展[J]. 中国烟草学报, 2014(3):113-118.
[10] Yan N, Du Y M, Liu X M , et al. Chemical structures, biosynjournal, bioactivities,biocatalysis and semisynjournal of tobacco cembranoids: an overview[J]. Industrial Crops & Products, 2016,83:66-80.
[11] Severson R F, Johnson A W, Jackson D M . Cuticular constituents of tobacco: factorsaffecting their production and their role in insect and diseaseresistance and smoke quality[J]. Recent Advances in TobaccoScience, 1985,11:105-174.
[12] 贾春晓, 何峰, 马宇平 , 等. 西柏烯类化合物研究进展[J]. 轻工学报, 2016,31(1):46-54.
[13] Ren X, He X F, Zhang Z F , et al. Isolation, identification, and autotoxicity effect of allelochemicals from rhizosphere soils of flue-cured tobacco[J]. Journal of Agricultural Food Chemistry, 2015,63(41):8975-8980.
[14] 冯精兰, 申君惠, 翟梦晓 , 等. 超声萃取-高效液相色谱测定沉积物中的多环芳烃[J]. 河南师范大学学报(自然科学版), 2011,161(6):88-91.
[15] 徐晖, 吴明红, 徐刚 . 高效液相色谱-串联质谱法对水环境中 12 种抗生素的检测[J]. 上海大学学报(自然科学版), 2017,23(3):483-490.
[16] Gao W, Wu N Y, Sun W L . Development and validation of a gaschromatography/mass spectrometry method for the determination ofalpha- and beta-2, 7, 11-cembratriene-4, 6-diols in tobacco[J]. World Journal of Engineering, 2016,13(4):341-347.
Options
Outlines

/

〈 〉