在线液相色谱-气相色谱/质谱联用快速分析烟叶中的西柏三烯二醇

doi:10.12066/j.issn.1007-2861.2078

Abstract

Abstract:

Cembratriene-diols are the precursors of important neutral aroma components and their derivatives, which affect significantly the aroma style and tobacco quality. In this study, a method of in-series combination of ultrasound-assisted extraction and online liquid chromatography-gas chromatography/mass spectrometry (LC-GC/MS) was constructed for the analysis of cembratriene-diols in tobacco. Cembratriene-diols were first extracted by ultrasound-assisted extraction and then on-line clean-up and analysis by online LC-GC/MS system. The pretreatment was highly efficient and easy-handling. Good recovery and reproducibility had also been achieved. This method was subsequently applied to the analysis of cembratriene-diols extracted from different regions of tobacco leaves. The satisfactory performance indicated that this strategy was suitable for the high-throughput analysis of cembratriene-diols in tobacco.

Key words: tobacco leaf, cembratriene-diols, silylation derivatization, liquid chromatography-gas chromatography/mass spectrometry (LC-GC/MS)

CLC Number:

O657.63

HUANG Zhi, BI Yanjiu, SHA Yunfei, XIE Wenyan, WU Da, LIU Baizhan, LIU Taiang. Analysis of cembratriene-diols in tobacco by LC-GC/MS[J]. Journal of Shanghai University（Natural Science Edition）, 2020, 26(2): 227-233.

Figures/Tables 7

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References 16

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Analysis of cembratriene-diols in tobacco by LC-GC/MS

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