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Journal of Shanghai University >

2021 , Vol. 27 >Issue 1: 97 - 105

DOI: https://doi.org/10.12066/j.issn.1007-2861.2085

Research Articles

Community structure and diversity phytoplankton in the Wujiang River Basin reservoir

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  • 1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
    2. Institute of Surface-Earth System Science, Tianjin University, Tianjin 300072, China

Received date: 2018-07-09

  Online published: 2018-09-14

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Abstract

Phytoplankton plays a crucial role in river ecosystems. To characterise the structure of phytoplankton communities in cascade reservoirs of the Wujiang River Basin, filter samples were collected at 0, 10, and 45 m depth, and two previously reported degenerate primers were applied to amplify the 23S rRNA gene fragment and construct the library for high-throughput sequencing. An efficient identification method was developed through comparison of species coverage between two degenerate primers, and primary information was obtained on the phytoplankton community diversity. This study provides a powerful tool for potential studies on the ecological effect of damming on phytoplankton communities in riverine ecosystems.

Key words： Wujiang River Basin; phytoplankton; degenerate primer; high-throughput sequencing

Cite this article

YUE Yihong, FU Zhiwei, CHEN Xueping, YANG Ming, WANG Baoli, WANG Fushun . Community structure and diversity phytoplankton in the Wujiang River Basin reservoir[J]. Journal of Shanghai University, 2021 , 27(1) : 97 -105 . DOI: 10.12066/j.issn.1007-2861.2085

References

[1] 苏维词. 乌江流域梯级开发的不良环境效应[J]. 长江流域资源与环境, 2002,11(4):93-97.
[1] Su W C. Adverse environmental effects of cascade development in Wujiang River Basin[J]. Resources and Environment in the Yangtze Basin, 2002,11(4):93-97.
[2] 冷刚, 顾文钰, 杜敦萌, 等. 乌江流域水资源综合利用面临形势及对策分析[J]. 科技展望, 2017,27(3):244-246.
[2] Leng G, Gu W Y, Du D M, et al. Analysis on the situation and countermeasures of the comprehensive utilization of water resources in Wujiang River Basin[J]. Science and Technology, 2017,27(03):244-246.
[3] 聂勇勇, 曹威. 浅析乌江流域水资源综合开发利用[J]. 科技展望, 2016,26(36):222-224.
[3] Nie Y Y, Cao W. A brief analysis of the comprehensive development and utilization of water resources in Wujiang River Basin[J]. Science and Technology, 2016,26(36):222-224.
[4] 庞峰. 乌江梯级水电站在贵州西电东送中的作用[J]. 水利水电技术, 2005,36(9):11-13.
[4] Pang F. The role of Wujiang cascade hydropower station in power transmission from west to east in Guizhou Province[J]. Water Resources and Hydropower Engineering, 2005,36(9):11-13.
[5] Kotut K, Krienitz L, Muthuri F M. Temporal changes in phytoplankton structure and composition at the Turkwel Gorge Reservoir, Kenya[J]. Hydrobiologia, 1998,368(1/3):41-59.
[6] Falkowski P G, Laws E A, Barber R T, et al. Phytoplankton and their role in primary, new, and export production[M]. Heidelberg: Springer, 2003: 99-121.
[7] Edlund M B. Freshwater algae of North America: ecology and classification[J]. Journal of Phycology, 2003,39(3):624-625.
[8] 刘建康. 高级水生生物学 [M]. 北京: 科学出版社, 1999.
[8] Liu J K. Advanced aquatic biology [M]. Beijing: Science Press, 1999.
[9] 赵文. 刺参池塘养殖生态学及健康养殖理论 [M]. 北京: 科学出版社, 2009: 109.
[9] Zhao W. Aquacultural ecology and health culture theory of sea cucumber in ponds [M]. Beijing: Science Press, 2009: 109.
[10] 黄良民, 张偲, 王汉奎, 等. 三亚湾生态环境与生物资源 [M]. 北京: 科学出版社, 2007: 105.
[10] Huang L M, Zhang S, Wang H K. Ecological environment and biological resources in Sanya bay [M]. Beijing: Science Press, 2007: 105.
[11] Zhang J H, Chung T D Y, Oldenburg K R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays[J]. Journal of Biomolecular Screening, 1999,4(2):67-73.
[12] Shendure J, Ji H. Next-generation DNA sequencing[J]. Nature Biotechnology, 2008,26(10):1135-1145.
[13] Zhan A, Hulák M, Sylvester F, et al. High sensitivity of 454 pyrosequencing for detection of rare species in aquatic communities[J]. Methods in Ecology & Evolution, 2013,4(6):558-565.
[14] Raes J, Bork P. Molecular eco-systems biology: towards an understanding of community function[J]. Nature reviews Microbiology, 2008,6(9):693-699.
[15] Yoon T H, Kang H E, Kang C K, et al. Development of a cost-effective metabarcoding strategy for analysis of the marine phytoplankton community[J]. PeerJ, 2016,4(6):e2115.
[16] Liu X L, Hou W G, Dong H L, et al. Distribution and diversity of cyanobacteria and eukaryotic algae in Qinghai-Tibetan Lakes[J]. Geomicrobiology Journal, 2016,33(10):860-869.
[17] 王崇, 朱代宏, 憨雪莹, 等. 乌江流域浮游植物群落结构的特征[J]. 水产学杂志, 2015,28(1):5-10.
[17] Wang C, Zhu D H, Han X Y, et al. Community structure characteristics of phytoplankton in Wujiang River[J]. Chinese Journal of Fisheries, 2015,28(1):5-10.
[18] 任启飞, 李荔, 王叁, 等. 贵州红枫湖水库浮游植物群落结构及优势种的季节性变化[C] // 庆祝中国藻类学会成立 30 周年暨第十五次学术讨论会摘要集. 2009.
[18] Ren Q F, Li L, Wang S, et al. The community structure of phytoplankotn and the seasonal variation of dominant species in Hongfeng Reservoir, Guizhou[C] // Celebration the 30th Anniversary of the Chinese Society of Algae and the 15th Symposium Summary. 2009.
[19] 王叁, 李荔, 任启飞, 等. 基于CCA 的喀斯特水库浮游植物群落动态特征分析------以百花湖水库为例[C] // 庆祝中国藻类学会成立 30 周年暨第十五次学术讨论会摘要集. 2009.
[19] Wang S, Li L, Ren Q F, et al. Structure and dynamics of phytoplankton community based CCA in a Karst Reservoir---as Baihua Resevroir[C] // Celebration the 30th Anniversary of the Chinese Society of Algae and the 15th Symposium Summary. 2009.
[20] 武秀国, 苏彦平, 陈修报, 等. 不同养殖类型池塘藻类群落特征[J]. 江苏农业科学, 2015,43(1):227-230.
[20] Wu X G, Su Y P, Chen X B, et al. Characteristics of algae community in different culture types[J]. Jiangsu Agricultural Sciences, 2015,43(1):227-230.
[21] 施择, 李爱军, 张榆霞, 等. 滇池浮游藻类群落构成调查[J]. 中国环境监测, 2014,30(5):121-124.
[21] Shi Z, Li A J, Zhang Y X, et al. Research on phytoplankton community of Dianchi Lake[J]. Environmental Monitoring in China, 2014,30(5):121-124.
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