乌江流域某水库浮游藻类群落结构及多样性分析

doi:10.12066/j.issn.1007-2861.2085

Abstract

Abstract:

Phytoplankton plays a crucial role in river ecosystems. To characterise the structure of phytoplankton communities in cascade reservoirs of the Wujiang River Basin, filter samples were collected at 0, 10, and 45 m depth, and two previously reported degenerate primers were applied to amplify the 23S rRNA gene fragment and construct the library for high-throughput sequencing. An efficient identification method was developed through comparison of species coverage between two degenerate primers, and primary information was obtained on the phytoplankton community diversity. This study provides a powerful tool for potential studies on the ecological effect of damming on phytoplankton communities in riverine ecosystems.

Key words: Wujiang River Basin, phytoplankton, degenerate primer, high-throughput sequencing

CLC Number:

Q178.51

YUE Yihong, FU Zhiwei, CHEN Xueping, YANG Ming, WANG Baoli, WANG Fushun. Community structure and diversity phytoplankton in the Wujiang River Basin reservoir[J]. Journal of Shanghai University（Natural Science Edition）, 2021, 27(1): 97-105.

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Community structure and diversity phytoplankton in the Wujiang River Basin reservoir

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