Abstract:

The matrix used in cell culture cannot match the requirement during the cell migration research in vitro at present. The three-dimentional collagen-matrigel mixture gel was studied which was made up of culture medium∶collagen∶matrigel by different ratios. The mixture gel got solidified within half an hour at room temperature, and did not show visible particles under microscope observation after fixation. The results from co-culture of cell aggregate and upper rhombic lip (URL) explant indicated that SDF-1 has the chemoattraction to URL cells, while slit has the chemorepulsion to them. 

Key words: three-dimentional mixture gel; cell migration; chemotaxis; SDF-1; slit