Abstract:

Based on resources of the traditional Chinese medicine (TCM) and large amount of prescriptions for diabetic treatment, 52 medicines were selected, and their aqueous extracts, ethanol extracts (60%~70%) of residues after aqueous extracts and ethanol extracts were tested respectively using α-glucosidase activity assays. Their α-glucosidase inhibition effect from rat small intestines and yeast are compared. TCM including mulberry stalk and raspberry have remarkable inhibition effect on rat’s small intestines and yeast at the concentration of 1 mg·mL-1. Four TCM (thanol extracts of residues after aqueous extracts) including Cortex Mori have strong inhibition both on yeast and ratintestines α-glucosidases. Kinetics of enzyme-catalyzed reactions of Cortex Mori was taken. The inhibition type is noncompetitive inhibition, Km=4.352×10-4 mmol·L-1. The results are valuable in research and discovery of new medicines for treatment of diabetes mellitus.

Key words: α-glucosidase; inhibitior; screening; traditional Chinese medicine (TCM); diabetes mellitus