Abstract: The recombinant coat protein of TMVSc1754 can induce hypersensitive reaction in susceptible tobacco. However this protein hardly is accumulated in the inoculated plants. In order to clarify the role of Sc1754CP in this hypersensitive reaction, we overexpressed Sc1754CP in E.coli. Polyclonal AntiSc1754CP rabbit polyclonal antibody has been successfully prepared. The Sc1754CP protein and the antibody against Sc1754CP can help us further study this unknown incompatible reaction between pathogen and host.

Key words: polyclonal antibody, prokaryotic expression, Sc1754CP