Abstract: Bisphenol A (BPA) and its analogues as main raw materials for synthesis of polycarbonate are important issue of environmental pollution. Most of the current studies focus on PBA analogues’ environmental distribution and human exposure. To evaluate the cytotoxic effects of BPA analogues, MTT assay was used to assess the proliferation toxicity of 7 kinds of BPA analogues on MCF-7 (ER–), when ER is estrogen receptor. At the same time, releases of lactate dehydrogenase (LDH) escaping into cell culture medium were detected to evaluate the damage of cell membrane. The results showed that in the concentration ranges of 0.01～1.00 μmol/L, the toxic effects of tested compounds on MCF-7 cells were small and only a small amount of LDH were released. At concentrations of 10 and 100 μmol/L, cell proliferation was significantly inhibited. Permeability of cell membranewas greatly changed, and lots of LDH were released. In addition, ER gene two-hybrid yeast bioassay was used to evaluate the estrogen effects of BPA analogues. All the dose-response relationships were described by logistic function. The estrogenic activity expressed by EC50 were TDP>BPAF>4Cl-BPA>BPC>BPA>BPE>BPAP. The results shows that the
estrogenic effect mechanism is due to the binding of that target compounds and ER.

Key words: bisphenol A (BPA) analogues, cell toxicity, estrogenic effect, MCF-7 cells