Journal of Shanghai University(Natural Science Edition) ›› 2015, Vol. 21 ›› Issue (2): 220-228.doi: 10.3969/j.issn.1007-2861.2014.03.001

• Metallurgical Materials • Previous Articles     Next Articles

Optimizing extraction technology of total flavonoids in Cynanchum wilfordii and its antioxidant effects

YANG Shen-ming1,2, WANG Ying-shun1,2, WANG Zhen-ji1,2   

  1. (1. Department of Chemistry and Life Science, Chuxiong Normal University,Chuxiong 675000, Yunnan, China;2. Yunnan Province Universities Key Laboratory of Applied Biology, Chuxiong 675000, Yunnan, China)
  • Received:2013-10-24 Online:2015-04-29 Published:2015-04-29

Abstract: Total flavonoids in Cynanchum wilfordii were extracted using an ethanol refluxing method, and the extraction procedure was optimized with orthogonal experiments. The antioxidant effects of the obtained total flavonoids on active oxygen radicals were studied. The results show the following optimum extraction parameters. The volume fraction of ethanol as extractant is 60%, the extraction temperature is 75℃, the extraction time is 60 min, and the ratio of solid to liquid is 1∶30 (g/mL). Under these conditions, the average extraction rate of total flavonoids reaches 10.79%, the average recovery rate of the added sample is 98.78%, and the scavenging effects of total flavonoids on ·OH and O2 · are 29.2% and 36.7%, respectively, implying that the material has good ability to scavenge ·OH and O2 ·.

Key words: Cynanchum wilfordii, hydroxyl radical, orthogonal experiment, superoxide anion free radical, total flavonoid, Cynanchum wilfordii, hydroxyl radical, orthogonal experiment, superoxide anion free radical, total flavonoid

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