Journal of Shanghai University(Natural Science Edition) ›› 2010, Vol. 16 ›› Issue (6): 562-566.doi: 10.3969/j.issn.1007-2861.2010.
• Environmental and Chemical Engineering • Previous Articles Next Articles
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Abstract:
Genomic DNA was extracted from a male Japanese medaka (Oryzias latipes) and a DNA fragment of 1 908 bp in length was cloned using specific primers by polymerase chain reaction (PCR) amplification. DMY- gene was then identified after sequencing, which consisted of three exons and two introns and encoded a peptide of 150 amino acids. In our study, an accurate and effective method was established for sex identification (gene type) of any development stage of medaka, which is amplifying household βactin gene to testify the DNA template and PCR amplification, and then amplifying specific DMY-gene to distinguish the gender of medaka. The method has been successfully applied to sex identification in randomly selected mature medaka and new born fish eggs.
Key words: Japanese medaka (Oryzias latipes); sex determination gene; DMY-gene
CLC Number:
X 17
YANG Ming, WANG Kun-Peng, ZHANG Yu-Ying, XU Gang, WU Meng-Hong. Gene Cloning and Application of DMY-Gene for Sex Identification in Oryzias latipes[J]. Journal of Shanghai University(Natural Science Edition), 2010, 16(6): 562-566.
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URL: https://www.journal.shu.edu.cn/EN/10.3969/j.issn.1007-2861.2010.
https://www.journal.shu.edu.cn/EN/Y2010/V16/I6/562