降低RNA结合蛋白FUS通过抑制circ-ZNF609减轻心肌细胞凋亡

doi:10.12066/j.issn.1007-2861.2720

Abstract

Abstract: This paper aims to investigate the regulatory role of RNA binding protein (RBP) fused in sarcoma (FUS) in the mechanism by which circ-ZNF609 (circBase ID: mmu_circ_0001797) alleviates cardiomyocyte apoptosis caused by ischemia/reperfusion injury (I/RI). Bioinformatics analysis revealed that FUS was a potential splicing factor binding to the flanking reverse complementary sequences of circ-ZNF609. Subsequently, an I/RI animal model and an oxygen-glucose deprivation/recovery (OGD/R) cell model were constructed. Methods such as terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were employed to validate that FUS can regulate the biogenesis of circ-ZNF609 in myocardial cells. The protective effect of FUS on OGD/R-induced cardiomyocyte apoptosis is reversed by circ-ZNF609 overexpression. Therefore, inhibiting FUS is a promising therapeutic strategy against the pathological process of myocardial ischemia/reperfusion injury.

Key words: fused in sarcoma (FUS), circular RNA, circ-ZNF609, cardiomyocyte apoptosis

CLC Number:

R541

BI Shenyan, CHEN Linghan, YU Pujiao, WANG Jiaqi, WANG Lijun, XU Jiahong. Alleviation of cardiomyocyte apoptosis by reducing RNA binding protein FUS and inhibiting circ-ZNF609[J]. Journal of Shanghai University（Natural Science Edition）, 2026, 32(2): 202-211.

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Alleviation of cardiomyocyte apoptosis by reducing RNA binding protein FUS and inhibiting circ-ZNF609

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