Journal of Shanghai University(Natural Science Edition) ›› 2021, Vol. 27 ›› Issue (1): 106-116.doi: 10.12066/j.issn.1007-2861.2065

• Research Articles • Previous Articles     Next Articles

Experimental methods for characterizing protein-protein interactions

KONG Wenna1,2, ZHOU Mi2, LIN Haixia1(), WANG Renxiao2   

  1. 1. College of Sciences, Shanghai University, Shanghai 200444, China
    2. State Key Laboratory of Bioorganic Chemistry and Natural Products, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
  • Received:2018-05-08 Online:2021-02-28 Published:2020-01-30
  • Contact: LIN Haixia E-mail:haixialin@staff.shu.edu.cn

Abstract:

Protein molecules usually interact with other partner protein molecules to execute their biological functions. Hence, the characterisation of protein-protein interac- tions (PPIs) plays a significant role inelucidating the molecular mechanism of proteins. With the progress of proteomics comes the development of a variety of experimental methods for characterising protein-protein interactions. This article mainly reviews recently developed biophysical- or biochemical-basedexperimental methods for characterising protein-protein interactions.

Key words: protein-protein interactions (PPIs), biochemical methods, biophysical methods

CLC Number: