高效液相色谱-串联质谱法对水环境中12种抗生素的检测

doi:10.12066/j.issn.1007-2861.1655

Abstract

Abstract:

This paper proposes a quantitative method for trace detection of 12 antibiotics including sulfonamides, β-lactams, fluoroquinolones, tetracyclines, macrolides in waters using solid phase extraction (SPE) coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS). After enriching thought hydrophile lipophilic balance (HLB) cartridges, 12 antibiotics are detected by LC-MS/MS with the organic phase of acetonitrile and aqueous phase of ammonium acetate and 0.2% formic acid. The antibiotics are quantified with multiple reaction monitoring (MRM) mode by internal standard of ¹³C3-caffeine. Quantification limits for antibiotics are from 0.004 83 to 4.805 00 ng/L in river water and the recoveries ranged from 71% to 90%. The method is applied to detect antibiotics in Nanchen River, Shanghai. Mean concentrations of antibiotics are from 2.04 to 254.46 ng/L.

Key words: liquid chromatography-tandem mass spectrometry (LC-MS/MS), sample pretreatment, antibiotics

XU Hui, WU Minghong, XU Gang. Determination of 12 antibiotics in aqueous environment by high performance LC-MS/MS[J]. Journal of Shanghai University（Natural Science Edition）, 2017, 23(3): 483-490.

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Determination of 12 antibiotics in aqueous environment by high performance LC-MS/MS

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