收稿日期: 2016-10-07
网络出版日期: 2018-10-26
基金资助
国家自然科学基金资助项目(21077070);国家自然科学基金资助项目(21377080);上海市教委科研创新基金重点资助项目(11ZZ90)
Inhibition of endogenous H$_\textbf{2}$S concentrations in human hepatocyte L02 cells by 1-chloro-2-hydroxy-3-butene
Received date: 2016-10-07
Online published: 2018-10-26
1-氯-3-丁烯-2-醇(1-chloro-2-hydroxy-3-butene, CHB)是环境污染物1,3-丁二烯的一种代谢产物, 具有细胞毒性、遗传毒性和致突变性. H$_{2}$S是继 NO 和 CO 之后发现的第 3 种气体第二信使分子, 涉及多种生理功能, 其代谢异常与很多疾病存在密切联系. 研究了 CHB 对人胚肝 L02 细胞中内源性 H$_{2}$S 浓度的影响. 结果发现, CHB 可明显降低细胞内源性 H$_{2}$S 的浓度, 且降低程度取决于 CHB 浓度的高低. 因此, CHB 对内源性 H$_{2}$S 产生的抑制效应可能是其毒性的一种机理.
关键词： 1-氯-3-丁烯-2-醇; H$_{2}$S; 1,3-丁二烯; 毒性
张芳, 张新宇 . 1-氯-3-丁烯-2-醇对人胚肝 L02 细胞中内源性 H$_{\textbf{2}}$S 的抑制效应[J]. 上海大学学报(自然科学版), 2018 , 24(5) : 800 -806 . DOI: 10.12066/j.issn.1007-2861.1825
1-chloro-2-hydroxy-3-butene (CHB) is a metabolite of 1,3-butadiene, an environmental pollutant. CHB is cytotoxic, genotoxic, and mutagenic. H$_2$S is the third gasotransmitter following NO and CO, and is involved in a variety of physiological activities. Abnormal metabolism of H$_2$S is associated with many diseases. In the present study, the effect of CHB on the concentrations of endogenous H$_2$S in human hepatocyte L02 cells was investigated. It was observed that CHB could cause significant decreases in the concentrations of endogenous H$_2$S in the cells and the effect depended on CHB concentration. Thus, inhibition of endogenous H$_2$S production in cells by CHB may be a mechanism for toxicity of CHB, which can contribute to a better understanding of toxicity of 1,3-butadiene.
Key words： 1-chloro-2-hydroxy-3-butene (CHB); H$_2$S; 1,3-butadiene; toxicity
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